神经药理学报››2016,Vol. 6››Issue (5): 14-21.DOI:10.3969/j.issn.2095-1396.2016.05.002
• 研究论文 •下一篇
汪林芳,黄术兵,徐一达,崔春,申延琴
出版日期:
2016-10-26发布日期:
2017-01-03通讯作者:
申延琴,教授,硕士研究生导师;研究方向:神经退行性疾病;Tel&Fax:+86-510-85197596;E-mail:shenyanqin@jiangnan.edu.cn作者简介:
汪林芳,女,硕士研究生;研究方向:神经退行性疾病;E-mail:18226622733@163.com 黄术兵,男,硕士研究生;研究方向:神经退行性疾病;E-mail:joaisheng@163.com基金资助:
国家自然科学基金项目(No.81650007)
WANG Lin-fang,HUANG Shu-bing,XU Yi-da,CUI Chun,SHEN Yan-qin
Online:
2016-10-26Published:
2017-01-03Contact:
申延琴,教授,硕士研究生导师;研究方向:神经退行性疾病;Tel&Fax:+86-510-85197596;E-mail:shenyanqin@jiangnan.edu.cnAbout author:
汪林芳,女,硕士研究生;研究方向:神经退行性疾病;E-mail:18226622733@163.com 黄术兵,男,硕士研究生;研究方向:神经退行性疾病;E-mail:joaisheng@163.comSupported by:
国家自然科学基金项目(No.81650007)
摘要:目的:研究马钱苷对神经毒素1- 甲基-4- 苯基-1,2,3,6- 四氢吡啶(1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine,MPTP)诱导的PC12 细胞自噬的影响。方法:MPTP 作用于PC12 细胞,观察MPTP 在不同时间和不同浓度对PC12 细胞中自噬相关因子微管相关蛋白1 轻链3-β(microtubule-associated protein1light chain 3-β,LC3B)的表达影响,并以此为基础探索马钱苷对MPTP 诱导的细胞自噬的影响和其潜在的作用通路。结果:PC12 细胞经MPTP 处理12 h 后,LC3B 的表达到达最高水平,且MPTP 在100 μg·mL-1 浓度下作用最强,而马钱苷在200 μg·mL-1 的浓度时降低MPTP 诱导的LC3B 高表达,在400 μg·mL-1 的浓度时降低了溶酶体膜相关蛋白2(lysosome associated membrane protein 2,LAMP-2)溶酶体标记蛋白的表达。结论:MPTP 在100 μg·mL-1 浓度下作用12 h,PC12 细胞内自噬蛋白的表达增加。马钱苷可降低MPTP 诱导的PC12 细胞内自噬体水平,并可能通过增强溶酶体对自噬体的消化实现的。
汪林芳,黄术兵,徐一达,崔春,申延琴.马钱苷对MPTP 诱导的PC12 细胞自噬的作用研究[J]. 神经药理学报, 2016, 6(5): 14-21.
WANG Lin-fang,HUANG Shu-bing,XU Yi-da,CUI Chun,SHEN Yan-qin.Effects of Loganin on MPTP-Induced Autophagy in PC12 Cells[J]. Acta Neuropharmacologica, 2016, 6(5): 14-21.
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