神经药理学报››2021,Vol. 11››Issue (3): 1-4.DOI:10.3969/j.issn.2095-1396.2021.03.001

• 研究论文 •下一篇

新生大鼠单侧基底核出血对神经功能的影响观察

周绍雄,胡金平

  1. 1 铜鼓县人民医院儿内科,宜春,336200,中国
    2 宜春市人民医院儿内科,宜春,336200,中国
  • 出版日期:2021-06-26发布日期:2021-06-26
  • 通讯作者:胡金平,男,主任医师;研究方向:小儿内科;E-mail:13970526817@163.com
  • 作者简介:周绍雄,男,主治医师;研究方向:小儿内科;E-mail:1032119039@qq.com

Effect of Unilateral Basal Ganglia Hemorrhage on Neurological Function in Neonatal Rats

ZHOU Shao-xiong,HU Jin-ping

  1. 1 Department of Pediatrics,Tonggu People’s Hospital,Yichun,336200,China
    2 Department of Pediatrics,Yichun People’s Hospital,Yichun,336000,China
  • Online:2021-06-26Published:2021-06-26

摘要:

目的:探析新生大鼠单侧基底核出血对神经功能产生的影响。 方法:抽取出生第10 天的54 只新生大鼠,根据随机数字分组的原则随机分为正常对照组(normal control group,NC, n=18)、假手术组(sham operation group,SH, n=18)、脑出血组(cerebral hemorrhage group,CH, n=18),通过立体定位仪将25 μL 自体血注入大鼠左侧脑尾状核位置,完成脑基底核出血模型的建立工作。NC 组不采取任何处理干预,给予正常饲养,SH 组在大鼠左侧尾状核位置刺入微量注射泵针头,不注血,其他处理和CH 组相同。对比3 组大鼠不同时间点神经功能评分、白天开场实验结果、晚上开场实验结果等指标。 结果:大鼠在造模之后神经功能呈现不同程度损伤,SH 组大鼠有着相对轻微的神经受损症状,能在短时间内恢复,在手术后72 h 能全部康复;CH 组大鼠发生肌张力改变以及偏瘫的情况;与SH 组、NC 组相比,CH 组大鼠在d 1、d 3 的神经功能评分明显较高( P<0.05);SH 组、NC 组大鼠在d 1、d 3、d 7、d 14 的神经功能评分对比差异不大( P>0.05);和SH 组、NC 组大鼠白天开场实验结果相比,CH 组大鼠理毛次数、直立次数、穿越格子数等明显较多( P<0.05);和SH 组、NC 组大鼠晚上开场实验结果相比,CH 组大鼠直立次数、穿越格子数等明显较多( P<0.05)。 结论:大鼠在发育期会出现单侧基底核局灶性出血损伤症状,有着可代偿性、可修复性等基本特征,大部分不会出现严重神经运动功能障碍遗留的情况,但会引起非选择性注意水平降低、自主活动增多等症状。

关键词:脑卒中,新生大鼠,单侧基底核出血,神经功能

Abstract:

Objective:To explore the effect of unilateral basal ganglia hemorrhage on neural function in neonatal rats. Methods:Fifty four neonatal rats were randomly divided into normal control group (NC,n = 18),sham operation group (SH,n = 18) and intracerebral hemorrhage group (CH,n = 18). 25 μL autologous blood was injected into the left caudate nucleus to establish the model of basal ganglia hemorrhage. NC group was given normal feeding without any intervention. In SH group,microinjection pump needle was inserted into the left caudate nucleus without blood injection. Other treatments were the same as those in CH group. The neurological function score at different time points,the results of daytime opening experiment and evening opening experiment were compared among the three groups. Results:After modeling,the neurological function of rats showed different degrees of damage. SH group rats had relatively mild neurological damage symptoms,which could recover in a short time,and all recovered 72 hours after operation. The changes of muscle tension and hemiplegia occurred in CH group. Compared with SH group and NC group,the neurological function score of CH group was significantly higher on the 1st and 3rd day (P<0.05). There was no significant difference in neurological function scores between SH group and NC group on the 1st,3rd,7th and 14th day (P>0.05).Compared with SH group and NC group,CH group had more grooming times,upright times and crossing grid times (P<0.05). Compared with SH group and NC group,CH group had more upright times and crossing grid number (P<0.05). Conclusion:The development of rats will appear unilateral basal ganglia focal hemorrhage injury symptoms,with compensatory,repairable and other basic characteristics,and most of them will not appear serious neuromotor dysfunction left behind,but will cause non selective attention level decreased,autonomic activity increased and other symptoms.

Key words:stroke,neonatal rats,unilateral basal ganglia hemorrhage,neurological function

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