Objective：To investigate the correlation between the physicochemical properties，functions and gene polymorphisms of the protein encoded by the cluster of differentiation antigen 14（CD14） gene and cardiovascular disease，so as to provide basis for the prevention and treatment of cardiovascular disease. Methods：Plasma samples were collected from 75 patients with cardiovascular disease （including 42 male patients and 33 female patients），and 125 healthy controls （including 80 male patients and 45 female patients）. Genomic DNA was extracted from their venous blood. The polymorphism of - 159C / T （rs2569190） locus of CD14 gene was detected by polymerase chain reaction restriction fragment length polymorphism （PCR-RFLP），and the results of enzyme digestion were observed by electrophoresis. The correlation between the polymorphism of-159C/T and cardiovascular disease was detected by genotype frequency. The physical and chemical properties，structure，function and interaction of CD14 gene coding protein were analyzed by bioinformatics methods to demonstrate the relationship between CD14 gene coding protein and cardiovascular disease. Results：The distribution frequency of TT genotype of CD14-159C / T（rs2569190） in the diseased group was 56%，which was significantly higher than that in the normal control group （40.8%）. The difference was statistically significant（ P<0.05），and the other genotypes were not statistically significant （P>0.05）. CD14 is a secretory protein，located in the extracellular matrix，without transmembrane region and weak hydrophilicity. There were more irregular curls in the secondary structure of CD14 protein （41.87%）. CD14 interacts directly with 10 proteins and plays an important role in Toll like receptor 4 signaling pathway and MyD88 dependent toll like receptor signaling pathway. Conclusion：The-159C/T（ rs2569190） TT genotype of CD14 gene is closely related to the occurrence of cardiovascular disease. CD14 protein may play an important role in the occurrence and development of cardiovascular disease.