关键词:抗氧化活性,药物筛选,微孔板定量检测,2,2-二苯基-1-苦肼基自由基,羟自由基,氧化还原,脂质过氧化

Abstract:Objective:To establish a series of anti-oxidant models of microplate quantification suitable for the drug screening.Methods:By using SpectraMax M5 continuous spectrum enzyme sign reflectoscope reflector，the anti-oxidant microplate quantification experiment modles of anti-DPPH oxidation activity，elimination hydroxy free radical，reduction ability determination，and lipid peroxidation were established.Results:All of the anti-oxidant microplate quantification experiment modles had more common characteristices of the good stability，the fewer reagent types and quantity，simple operation and step，the good repeatability，and so on. Relatively speaking，the two anti-oxidant modles of anti-DPPH oxidation activity，reduction ability determination had more advantage in these experiment modles，and had more suitable for initially screening experiment of the anti-oxidant drug as basic research.Conclusion:These four modles of the anti-oxidant microplate quantification may be widely applied in anti-drug screening，especially high-throughput screening research.