黄芩茎叶总黄酮对Aβ<sub>25-35</sub>致大鼠学习记忆损伤及海马抗氧化酶活性的影响

神经药理学报››2011,Vol. 1››Issue (2): 14-18.

黄芩茎叶总黄酮对Aβ_25-35致大鼠学习记忆损伤及海马抗氧化酶活性的影响

王瑞婷¹，关丽华¹，周健¹，申兴斌²，张建新³

1. 承德医学院药理教研室，承德，067000，中国
2. 承德医学院附属医院，承德，067000，中国

3. 河北省医学科学院药物研究所，石家庄，050021，中国

出版日期:2011-04-26发布日期:2012-04-20
通讯作者:张建新，男，教授，博士生导师；研究方向：心脑血管药理学研究；Tel：+86-0311-86573288，E-mail：zhangjx100@163.com
作者简介:王瑞婷，女，博士，教授，硕士生导师，河北省药理学会常务理事；研究方向：中药抗痴呆作用及机制研究；Tel：+86-0314-2290069，E-mail:?wrtwrt8324@sina.com
基金资助:

河北省科技厅资助项目（No.08276101D-21）

Effect of Scutellaria Baicalensis Stem-leaf Total Flavonoid on Learning and Memory Impairment Induced by Aβ_25-35and Activity of Anti-oxidative Enzyme of Hippocampus in Rats

WANG Rui-ting¹, GUAN Li-hua¹, ZHOU Jian¹, SHEN Xing-bin²，ZHANG Jian-xin³

1. Department of Pharmacology, Chengde Medical College, Chengde, 067000，China
2. The Affiliated Hospital of Chengde Medical College, Chengde, 067000，China

3. Insititute Meteria Medica, Hebei Academy of Medical Sciences, Shijiazhuang, 050021, China

Online:2011-04-26Published:2012-04-20

摘要/Abstract

摘要：目的: 探讨黄芩茎叶总黄酮（scutellaria baicalensis stem-leaf total flavonoid, SSTF）对大鼠海马注射 Aβ_25-35致大鼠学习记忆损伤及海马抗氧化酶活性的影响。方法：给大鼠灌胃（ig）SSTF 100,50,25 mg• kg^-1剂量 , 采用双侧海马注射 Aβ_25-35制作大鼠痴呆模型，Morris 水迷宫实验测定大鼠学习、记忆能力；HE 染色观察大鼠海马 CA1 区神经元形态；检测海马组织中超氧化物歧化酶（superoxide dismutase,SOD）、谷胱甘肽过氧化物酶（glutathione peroxidase,GSH-Px）活性及丙二醛（malondialdehyde，MDA）含量。结果：模型组大鼠寻找平台潜伏期较对照组大鼠明显延长（P<0.01），2 min 内在平台象限所游路程与总路程的比值明显低于对照组（P<0.01）,SSTF 100,50 mg• kg^-1剂量组及维生素 E 组的大鼠寻找平台潜伏期较模型组明显缩短，所游路程比值明显高于模型组；模型组大鼠海马 CA1 区神经元损伤明显，SSTF100，50 mg• kg^-1剂量组及维生素 E 组大鼠海马神经元损伤较模型组明显减轻；SSTF 100 mg• kg^-1剂量组海马组织中 SOD、GSH-Px 活性较模型组明显升高，MDA 含量明显减少。结论：黄芩茎叶总黄酮能减轻大鼠海马注射 Aβ_25-35引起的海马神经元损伤，改善学习记忆能力，其机制可能与提高组织中抗氧化酶活性有关。

关键词:黄芩茎叶总黄酮,阿尔茨海默病,学习记忆,超氧化物歧化酶,谷胱甘肽过氧化物酶

Abstract:Objective: To explore the effect of scutellaria baicalensis stem-leaf total flavonoid（SSTF）on learning and memory damage induced by Aβ_25-35and on the activity of anti-oxidative enzyme.Methods：Rats were administered SSTF（100,50，25 mg• kg^-1，ig）. The AD model was made by bilateral injection of Aβ_25-35in rat hippocampus. Morris water maze was used to evaluate the learning and memory ability of the rats. HE stain was used to observe morphology of the neuron of hippocampus CA1 region. The activity of superoxide dismutase（SOD），glutathione peroxidase（GSH-Px）and the content of malondialdehyde（MDA）in rat hippocampus were measured.Results: Compared with that of the control group, the latency to find the platform in model group was significantly increased, and the ratio of path in platform quadrant to the whole path was significantly decreased. Compared with that of the model rats, the latency to find the platformin animals with SSTF 100，50 mg• kg^-1and vitamin E （Vit E）groups were significantly decreased, the ratio of path in platform quadrant to the whole path was significantly increased. The CA1 region neuron of the model rats showed severe damage. Compared with of the model rats, the neuron of SSTF and Vit E groups showed a decreased damage. Activities of SOD, GSH-Px in SSTF and Vit E group were significantly increased compared with that in the model rats. Content of MDA in SSTF and Vit E groups were significantly decreased compared with that in the model rats.Conclusion: SSTF can attenuate neuron damage and learning impairment induced by Aβ, and the mechanism may be related to the increased activity of anti-oxidative enzyme.

Key words:scutellaria baicalensis stem-leaf total flavonoid,Alzheimer&,rsquo,s disease,learning and memory,superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)

中图分类号:

R962

王瑞婷，关丽华，周健，申兴斌，张建新. 黄芩茎叶总黄酮对Aβ_25-35致大鼠学习记忆损伤及海马抗氧化酶活性的影响[J]. 神经药理学报, 2011, 1(2): 14-18.

WANG Rui-ting, GUAN Li-hua, ZHOU Jian, SHEN Xing-bin，ZHANG Jian-xin. Effect of Scutellaria Baicalensis Stem-leaf Total Flavonoid on Learning and Memory Impairment Induced by Aβ_25-35and Activity of Anti-oxidative Enzyme of Hippocampus in Rats[J]. Acta Neuropharmacologica, 2011, 1(2): 14-18.

参考文献

1 Deborah J Tew，Stephen P Bottomley, David P Smith, et al. Stabilization of neurotoxic soluble beta-sheet-rich conformations of the Alzheimer’s disease amyloid-beta peptide[J]. Biophys J, 2008, 94(7)：2752-2766.

2 Kelly BL, Ferreira A. Beta-amyloid disrupted synaptic vesicle endocytosis in cultured hippocampal neurons[J]. Neuroscience, 2007, 147(1)：60-70.

3 Ge Yu-song, Teng Wei-yu , Zhang chao-dong. Protective effect of cyclophilin A against Alzheimer′s amyloid beta-peptide (25-35)-induced oxidative stress in PC12 cells[J]. Chin Med J (Engl), 2009 , 122(6)：716-724.

4 Angara Zambrano, Carola Otth, Lorena Mujica, et al. Interleukin-3 prevents neuronal death induced by amyloid peptide[J]. BMC Neurosci,2007,8(1)：82-85.

5 赵淑敏，刘胜，杨宏光. 黄芩茎叶总黄酮预处理对缺血再灌注心肌脂质过氧化损伤[J]. 解剖学杂志, 2006，29（4）：450-452.

6 Morimoto K, Yoshimi K, Tonohiro T, et al. Co-injection of β-amyloid with ibotenic acid induces synergistic loss of rat hippocampal neurons[J]. Neuroscience, 1998, 84(2)：479-87.

7 Lu P, Mamiva P, Lu LL, et al. Silibinin attenuates amyloid {beta}25-35 peptide-induced memory impairments: Implication of inducible nitricoxide synthase (iNOS) and tumor necrosis factor-alpha (TNF-{alpha}) in mice[J]. J Pharmacol EXP Ther, 2009,331(1)：319-326.

8 Rodrigo Medeiros, Rui D.S. Prediger, Giselle F Passos, et al. Connecting TNF-α signaling pathways to iNOS expression in a mouse model of Alzheimer’s disease: Relevance for the behavioral and synaptic deficits induced by amyloid β protein[J]. J Neurosci，2007,27 (20)：5394-5404.

9 Amit Berson, Marlen Knobloch, Mor Hanan, et al. Changes in readthrough acetylcholinesterase expression modulate amyloid-beta pathology[J]. Brain,2008,131(1)：109-119.

10 王瑞婷，张建新，董雅洁. 黄芩茎叶总黄酮对AD大鼠模型海马神经元超微结构的影响及抗氧化作用[J]. 中国老年学杂志, 2010,30（7）：926-928.

11 王瑞婷，申兴斌，周健. 黄芩茎叶总黄酮对AD大鼠海马神经元的保护作用及机制研究[J]. 中国新药杂志, 2010,19（11）：982-985.

12 汪保华，袁华，吕志华. 灵芝多糖对模拟AD学习记忆障碍大鼠海马白细胞介素-6表达的影响[J].卒中与神经疾病，2007,14（4）：206-209.

13 苗青，阎福岭. 维生素E对AD大鼠海马HNE含量的影响[J]. 解剖与临床，2007,12（2）：98-100.

14 Yazhen Shang , Jianjun Cheng, Jiemin Qi, et al. Scutellaria flavonoid reduced memory dysfunction and neuronal injury caused by permanent global ischemia in rats[J]. Pharmacol BiochemI be, 2005,82(1)：67-73.

15 Hafiz Mohmmad Abdul, Rukhsana Sultana, Daret K. St. Clair, et al. Oxidative damage in brain from human mutant APP/PS-1 double knock-in mice as a function of age[J]. Free Radic Biol Med, 2008,45(10)：1420-1425.

16 Yoichiro Nishida , Shingo Ito ,Sumio Ohtsuki , et al. Depletion of vitamin E increases Abeta accumulation by decreasing its clearances from brain and blood in a mouse model of Alzheimer’s disease[J]. J Biol Chem, 2009,284(48)：33400-33408.

17 Elena Tamagno, Michela Guglielmotto, Manuela Aragno, et al. Oxidative stress activates a positive feedback between the γ- and β-secretase cleavages of the β-amyloid precursor protein[J]. J Neurochem, 2008 , 104 (3)：683-695.

18 陈秀敏，李西平，杨晓梅，等. 飞蓬属11种黄酮类化合物抗氧化活性的理论评价[J]. 计算机与应用化学，2009，26（7）：925-930.

19 冯星，许东晖，梅雪婷，等. 儿茶素和漆黄素对柠檬酸铁诱导大鼠原代肝细胞损伤的保护作用[J]. 中国药理学通报，2003，19(12)：1397-1401.

20 Afolabi C Akinmoladun , Efere M Obuotor , Ebenezer O Farombi . Evaluation of Antioxidant and Free Radical Scavenging Capacities of Some Nigerian Indigenous Medicinal Plants[J]. J Med Food, 2010, 13(2):444-451.

[1]	谢彬, 黄志源, 林多朵, 杨福龙, 谢奕彬.针药结合干预阿尔茨海默病抑郁症状效果分析[J]. 神经药理学报, 2020, 10(5): 5-8.
[2]	赵雨薇, 甄艳杰, 戴月英, 沈丽霞.槲皮素对阿尔茨海默症神经保护作用研究[J]. 神经药理学报, 2020, 10(5): 55-64.
[3]	海吉涛, 罗焕敏.病原微生物与阿尔茨海默病相关性研究进展[J]. 神经药理学报, 2020, 10(4): 58-64.
[4]	杨旭华, 杜爽, 沈丽霞, 郝军荣.阿尔茨海默病的药物治疗研究进展[J]. 神经药理学报, 2020, 10(3): 47-53.
[5]	甄艳杰, 郭童林, 赵雨薇, 沈丽霞.植物雌激素介导线粒体途径对阿尔茨海默病神经保护作用的研究进展[J]. 神经药理学报, 2020, 10(1): 40-46.
[6]	杨琳，艾静.脑源雌激素在阿尔茨海默病中的作用研究进展[J]. 神经药理学报, 2019, 9(5): 50-64.
[7]	张帅，艾静.谷氨酸功能异常与阿尔茨海默病[J]. 神经药理学报, 2018, 8(6): 9-20.
[8]	SHEN li-xia1，LIU Liang-liang1，ZHANG Ming1，LIU Yang1，ZHANG Dan-shen 2*.Research of Quercetin’s Estrogen-Like Action on Central Nervous System and Its Mechanisms[J]. 神经药理学报, 2018, 8(4): 23-25.
[9]	黄蕊，杨翠翠，张兰.二苯乙烯苷对APP/PS1 双转基因小鼠学习记忆及突触可塑性的影响[J]. 神经药理学报, 2018, 8(2): 31-31.
[10]	雷曦，王健辉，程肖蕊，张小锐，刘港，周文霞，张永祥.基于快速老化模型小鼠SAMP8 的CA-30 抗阿尔茨海默病的作用研究[J]. 神经药理学报, 2018, 8(2): 50-50.
[11]	王健辉，程肖蕊，张小锐，刘港，周文霞，张永祥.药物组合吲哚美辛与阿托伐他汀对阿尔茨海默病的治疗作用研究[J]. 神经药理学报, 2018, 8(2): 52-52.
[12]	王静，程肖蕊，周文霞，张永祥.快速老化模型小鼠海马囊泡谷氨酸转运体表达与兴奋性毒性关系的研究[J]. 神经药理学报, 2018, 8(2): 53-53.
[13]	张林，蒋宁，周文霞，张永祥.疾病特异性诱导性多功能干细胞AD 模型建立[J]. 神经药理学报, 2018, 8(2): 54-54.
[14]	郭鹏，张巍.阿尔茨海默病患者睡眠障碍及其与认知障碍关系的研究[J]. 神经药理学报, 2018, 8(2): 61-61.
[15]	连腾宏，李少武，余秋瑾，等.阿尔茨海默病伴发嗅觉障碍的临床特点及静息态功能核磁共振成像的研究[J]. 神经药理学报, 2018, 8(2): 62-62.

黄芩茎叶总黄酮对Aβ_25-35致大鼠学习记忆损伤及海马抗氧化酶活性的影响

Effect of Scutellaria Baicalensis Stem-leaf Total Flavonoid on Learning and Memory Impairment Induced by Aβ_25-35and Activity of Anti-oxidative Enzyme of Hippocampus in Rats

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章15

编辑推荐

Metrics

黄芩茎叶总黄酮对Aβ25-35致大鼠学习记忆损伤及海马抗氧化酶活性的影响

Effect of Scutellaria Baicalensis Stem-leaf Total Flavonoid on Learning and Memory Impairment Induced by Aβ25-35and Activity of Anti-oxidative Enzyme of Hippocampus in Rats

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章15

编辑推荐

Metrics

黄芩茎叶总黄酮对Aβ_25-35致大鼠学习记忆损伤及海马抗氧化酶活性的影响

Effect of Scutellaria Baicalensis Stem-leaf Total Flavonoid on Learning and Memory Impairment Induced by Aβ_25-35and Activity of Anti-oxidative Enzyme of Hippocampus in Rats