高压静电法制备微胶囊化人视网膜色素上皮细胞

神经药理学报››2011,Vol. 1››Issue (2): 24-27.

高压静电法制备微胶囊化人视网膜色素上皮细胞

高冬晓，赵亚群

中国人民解放军第三0九医院神经外科，北京，100091，中国

出版日期:2011-04-26发布日期:2012-04-20
通讯作者:赵亚群，男，硕士，副主任医师，硕士生导师；研究方向：立体定向神经外科；Tel：+86-010-66775066，E-mail：zhaoyaqun2@sina.com
作者简介:高冬晓，女，硕士；研究方向：神经药理学；Tel：+86-010-66775066，E-mail:gaodongxiao08@yeah.net
基金资助:

北京市海淀区科委项目（No.0831）

Production of Microencapsulated Human Retinal Pigment Epithelial Cells by the High Voltage Electrostatic Method

GAO Dong-xiao，ZHAO Ya-qun

Department of Neurosurgery, 309^thHospital of PLA, Beijing, 100091, China

Online:2011-04-26Published:2012-04-20

摘要/Abstract

摘要：目的：探索用高压静电法制备人视网膜色素上皮细胞（human retinal pigment epithelial cell，hRPE）海藻酸钠-壳聚糖-海藻酸钠（Alginate-Chitosan-Alginate，ACA）微胶囊。方法：用自制的高压静电微胶囊发生装置制备包被hRPE细胞的ACA微胶囊，并观察所得细胞微胶囊在培养液中培养1周的细胞总数及存活率。结果：制备出直径为（400±13）μm的hRPE细胞ACA微胶囊。d 1~d 3微胶囊内细胞总数及存活率变化不显著，而培养到d 7后总数显著上升，存活率降低，但总的活细胞数增加。结论：用自制的高压静电微胶囊发生装置能成功制备出hRPE细胞的ACA微胶囊；包被在微胶囊中的细胞培养1周后，细胞增殖显著。

关键词:高压静电法,微胶囊化,人视网膜色素上皮细胞

Abstract:Objective: To prepare alginate-chitosan-alginate（Alginate-Chitosan-Alginate, ACA） microencapsulated Human Retinal Pigment Epithelial（hRPE） cells using high voltage electrostatic method.Methods: The microencapsulated hRPE was prepared by using our home-made high voltage electrostatic system, and the total number and the survival rate of microencapsulated hRPE cells were studied in the culture media for 7 d.Results: hRPE cells ACA microcapsule with diameter of （400±13） μm were obtained. the total number and the survival rate of cells had no statistically significant differences at the first 3 d. But after 7 d the total number of cells and the survival number were increased and the survival rate of cells was reduced.Conclusion: hRPE cells ACA microcapsule can be obtained with high voltage electrostatic method; after 7 d, the microencapsulated cells grow faster.

Key words:high voltage electrostatic preparation method,microencapsulated,human reti-nal pigment epithelial Cell（hRPE）

中图分类号:

高冬晓，赵亚群. 高压静电法制备微胶囊化人视网膜色素上皮细胞[J]. 神经药理学报, 2011, 1(2): 24-27.

GAO Dong-xiao，ZHAO Ya-qun. Production of Microencapsulated Human Retinal Pigment Epithelial Cells by the High Voltage Electrostatic Method[J]. Acta Neuropharmacologica, 2011, 1(2): 24-27.

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