<strong>蜂斗菜素抑制人神经母细胞瘤细胞SK-N-SH增殖作用机制研究</strong>

神经药理学报››2011,Vol. 1››Issue (3): 1-6.

• 研究论文 •下一篇

蜂斗菜素抑制人神经母细胞瘤细胞SK-N-SH增殖作用机制研究

程月芳，王彩芳，李文杰，林青，张旗

郑州大学药学院，郑州，450001，中国

出版日期:2011-06-26发布日期:2012-06-28
通讯作者:张旗，男，教授，硕士生导师；研究方向：神经精神药理学；Tel & Fax：+86-371-67781908
作者简介:程月芳，女，硕士研究生；研究方向：神经肿瘤药物；Tel：+86-371-67781908
基金资助:

国家自然科学基金项目（No.30800328），河南省高校科技创新人才项目

Antiproliferation Effects of Petasin on Human Neuroblastoma SK-N-SH Cells

Cheng Yue-fang, Wang Cai-fang, Li Wen-jie, Lin Qing, Zhang Qi

School of Pharmaceutical Sciences，Zhengzhou University

Online:2011-06-26Published:2012-06-28

摘要/Abstract

摘要：目的：探讨蜂斗菜素对人神经母细胞瘤细胞SK-N-SH增殖抑制作用的机制。方法：采用SRB法检测蜂斗菜素对肿瘤细胞的增殖抑制作用；采用流式细胞术观察蜂斗菜素对SK-N-SH细胞周期和凋亡的影响；采用Hochest 33258染色法观察蜂斗菜素对SK-N-SH凋亡的影响；蛋白免疫印迹法( Western Blotting ) 检测蜂斗菜素对SK-N-SH细胞ERK1/2蛋白磷酸化的影响。结果：蜂斗菜素对SK-N-SH，SGC7901，SPC-A-1，SMMC 7721，Ec9706，SK-N-BE(2)和U251细胞有不同程度的抑制作用。蜂斗菜素使SK-N-SH细胞的周期阻滞于G0/G1期，但不诱导其凋亡，且下调ERK1/2蛋白的磷酸化水平。结论：蜂斗菜素是一种潜在的抗肿瘤天然化合物，其机制为抑制细胞增殖，但不诱导其凋亡。其分子机制可能包括下调ERK1/2蛋白的磷酸化水平。

关键词:蜂斗菜素,SK-N-SH,细胞,增殖,ERK1/2

Abstract:Objective:To study the antiproliferation effects of petasin on human neuroblastoma SK-N-SH cells and the possible mechanisms involved.Methods:The inhibitory effects of petasin on proliferation of tumor cells was detected by SRB assay;the effects ofpetasin on cell cycle arrest of SK-N-SH were measured by flow cytometry; the effects of apoptosis were examined by Hochest 33258 staining and flow cytometry and thephosphorylationof ERK1/2 of SK-N-SHwas determined bywestern blotting.Results:Compared to that of other cancer cell lines such as SGC7901, SPC-A-1, SMMC 7721, Ec9706, SK-N-BE (2) and U251, the proliferation of SK-N-SH cells were strongly inhibited by petasin. After treated with petasin, the cell cycle of SK-N-SH arrested in G0/G1 phase, and there was no apoptosis detected either by Hoechst 33258 staining or by flow cytometry analysis. Finally, the phosphorylation of ERK1/2 was decreased by pretreatment with petasin in a dose-dependent way.Conclusion:Petasin is a potential anti-tumor of natural compounds, and shows the strong antiproliferation effect on SK-N-SH cells without inducing apoptosis. The inhibition of ERK1/2 phosphorylation might be involved in the antiproliferation effects of petasin.

Key words:petasin,SK-N-SH,cell,proliferation,ERK1/2

中图分类号:

R961

程月芳，王彩芳，李文杰，林青，张旗.蜂斗菜素抑制人神经母细胞瘤细胞SK-N-SH增殖作用机制研究[J]. 神经药理学报, 2011, 1(3): 1-6.

Cheng Yue-fang, Wang Cai-fang, Li Wen-jie, Lin Qing, Zhang Qi.Antiproliferation Effects of Petasin on Human Neuroblastoma SK-N-SH Cells[J]. Acta Neuropharmacologica, 2011, 1(3): 1-6.

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蜂斗菜素抑制人神经母细胞瘤细胞SK-N-SH增殖作用机制研究

Antiproliferation Effects of Petasin on Human Neuroblastoma SK-N-SH Cells

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