神经药理学报››2012,Vol. 2››Issue (6): 36-39.

• 实验方法学 •上一篇下一篇

正交试验优化南五味子多糖的超声提取工艺

谭晓虹,王治宝,卫娇娇,张亚飞,卢金铭

  1. 1 欧宝平台登录 药学系,张家口,075000,中国
    2 华北电力大学科技学院,保定,071000,中国
  • 出版日期:2012-12-26发布日期:2014-06-27
  • 作者简介:谭晓虹, 女,硕士,副教授,主要从事中药有效成分分析与药代动力学研究。E-mail :TXHZBWZL@ 126. com
  • 基金资助:

    河北省中医药管理局资助项目(No. 2013076)

Study on Extraction Polysaccharide from Fructus Schisandrae Sphenantherae by Orthogonal Ultrasound-Assisted Method

TAN Xiao-hong,WANG Zhi-bao,WEI Jiao-jiao,ZHANG Ya-fei,LU Jin-ming

  1. 1 Department of Pharmacy, Hebei North University, Zhangjiakou, 075000, China
    2 North China Electric Power University Science and Technology College, Baoding, 071000, China
  • Online:2012-12-26Published:2014-06-27
  • About author:谭晓虹, 女,硕士,副教授,主要从事中药有效成分分析与药代动力学研究。E-mail :TXHZBWZL@ 126. com
  • Supported by:

    河北省中医药管理局资助项目(No. 2013076)

摘要:目的:优化南五味子多糖的提取条件。方法:采用正交试验设计,以南五味子多糖提取率为考察指标,考察提取时间(extraction time)、固液比(solid-liquid ratio)和浸泡时间(immersion time)对多糖提取率的影响。结果:试验表明南五味子多糖提取所需的最优时间为40min,浸泡最佳时间为90min,固液比为1:25,在此条件下南五味子多糖提取率较高。结论:优选出的最佳提取条件,试验证实结果稳定,方法可行,为南五味子多糖的进一步研究提供参考。

关键词:南五味子,多糖,超声辅助,正交试验

Abstract:Objective: To optimize the extraction condition for polysaccharide of Fructus Schisandrae Sphenantherae.Methods:The extraction technique was designed by orthogonal test with extraction time,solid-liquid ratio and immersion time as factors using extraction rate as index.Results:The optimum conditions for the ultrasound-assisted method to extract polysaccharide were as follows: extraction time was 40min, solid-liquid ratio was 1:25, immersion time was 90min.Conclusion:The optimum process is feasible, stable and reasonable. It provide reference for the further study of the polysaccharide of Fructus Schisandrae

Key words:fructus schisandrae sphenantherae,polysaccharide,ultrasound-assisted,orthogonal test

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