体外诱导的神经元样细胞对大鼠脑损伤模型修复的影响

神经药理学报››2013,Vol. 3››Issue (6): 20-24.

体外诱导的神经元样细胞对大鼠脑损伤模型修复的影响

魏会平¹，朱登祥¹，张爱兰¹，宋小青¹，崔乐²

1.欧宝平台登录生物教研室，张家口，075000，中国
2.欧宝平台登录 09级检验2班，张家口， 075000，中国

出版日期:2013-12-26发布日期:2014-06-27
作者简介:魏会平，女，教授；研究方向：细胞生物学和遗传学；E-mail：whp123456@sina.com
基金资助:

国家自然科学基金课题(No.81274005),河北省张家口市科技局发展项目（No.0911021D-4）

Repairment ofIn VitroInduced Neuron-like Cells on the Brain Injury in Rats

WEI Hui-ping, ZHU Deng- xiang, ZHANG Ai-lan, SONG Xiao-qing, CUI Le

Hebei North University, Zhangjiakou, 075000, China

Online:2013-12-26Published:2014-06-27
About author:魏会平，女，教授；研究方向：细胞生物学和遗传学；E-mail：whp123456@sina.com
Supported by:

国家自然科学基金课题(No.81274005),河北省张家口市科技局发展项目（No.0911021D-4）

摘要/Abstract

摘要：目的:探讨大鼠骨髓间充质干细胞（bone marrow mesenchymal stem cells, BMSCs) 诱导为神经元样细胞后对大鼠脑损伤模型修复的影响。方法:骨髓贴壁法分离培养大鼠BMSCs并采用流式细胞术进行鉴定,川芎嗪诱导其分化，将诱导24h的细胞直接注射移植到大鼠脑缺血模型海马区组织, 检测分化的神经元样细胞在神经系统微环境中存活及病理变化。结果:细胞移植组病理变化与脑损伤模型组相比, 神经细胞变性、坏死数量明显减少, 间质水肿较轻。结论:骨髓间充质干细胞诱导为神经元样细胞后移植入对大鼠脑缺损伤的修复有一定的作用。

关键词:脑缺血模型,骨髓间充质干细胞,神经元样细胞

Abstract:Objective:To investigate the effect of neuron-like cells induced from bone marrow mesenchymal stem cells (BMSC) inthe repairment of brain injury in rats.Methods:BMSCs were isolated and cultured from rat's bone marrow by adherent method, identified by flow cytometry. BMSCs were induced into neuron-like cells using ligustrazine. 24 hrs after Neuron-like cell induction, the cells were directly transplanted into the hippocampus of rats with brain injury, and the survival of neuron-like cells in microenvironment of nervous system and pathological changes was examined.Results:Compared to rats with brain injury alone, rats that received neuron-like cells transplantation showed a decreased number of nerve cell degeneration and necrosis and less significant interstitial edema.Conclusion: rats with brain injury.Neuron-like cells induced from BMSC can improve brain function of

Key words:cerebral ischemia model,bone marrow mesenchymal stem cells(BMSCs),neuron-like cells

魏会平, 朱登祥, 张爱兰, 宋小青, 崔乐.体外诱导的神经元样细胞对大鼠脑损伤模型修复的影响[J]. 神经药理学报, 2013, 3(6): 20-24.

WEI Hui-ping, ZHU Deng- xiang, ZHANG Ai-lan, SONG Xiao-qing, CUI Le.Repairment ofIn VitroInduced Neuron-like Cells on the Brain Injury in Rats[J]. ACTA NEUROPHARMACOLOGICA, 2013, 3(6): 20-24.

参考文献

1. Wang T T, Tio M, Lee W, et al. Neural differentiation of mesenchymal-like stem cells from cord blood is mediated by PKA [J]. Biochem Biophys Res Commun, 2007, 357(4): 1021-1027.

2. Wei Li, Su-nan Liu, Duan-de Luo, et al. Differentiation of hepatocytoid cell induced from whole-bone-marrow method isolated rat myeloid mesenchymal stem cells [J]. World J Gastroenterol, 2006, 12(30): 4866-4968.

3. G. Ciapetti, L Ambrosio, G Marletta, et al. Human bone marrow stromal cells: In vitro expansion and differentiation for bone engineering [J]. Biomaterials, 2006, 27(36): 6150-6160.

4. P F Choong, P L Mok, S K Cheong, et al. Generating neuron-like cells from BM-derived mesenchymal stromal cells in vitro [J]. Cytotherapy, 2007, 9(2):170-183.

5. 郭亮, 麦晓燕, 申晶, 等. 骨髓间充质干细胞移植后在脑梗死大鼠脑内的存活与分化[J]. 中国组织工程研究与临床康复, 2008, 12(34): 6661-6664.

6. 郭亮, 李源, 宋祖军, 等. 骨髓间充质干细胞体外诱导分化为神经细胞的实验研究[J]. 中华老年心脑血管病杂志, 2005, 7(1): 50-52.

7. Nakajima Wako, Akira Ishida, Goro Takada, Anoxic and hypoxic immature rat model for measurement of monoamine using in vivo microdialysis [J]. Brain Res Brain Res Protoc, 1999, 3(3): 252-256.

8. 刘继云, 魏会平，李继红. 骨髓间充质干细胞诱导为神经样细胞后对大鼠脑缺血模型修复的研究[J]. 陕西医学杂志, 2007, 36(8): 949-951.

9. 余勤, 李佩佩, 宣晓波, 等. 间充质干细胞不同移植途径对修复大鼠缺氧缺血性脑损伤作用的研究[J]. 浙江中医药大学学报, 2012, 06: 696-700.

10. 杜晓鸣, 魏会平, 张爱兰, 等. 川芎嗪对大鼠骨髓间充质干细胞长期诱导效应的研究[J]. 中国中医药远程教育, 2012, 10(13): 157-158.

11. 姚玲, 汤永红. 骨髓间充质干细胞移植修复缺血性脑损伤[J]. 社区医学杂志, 2009, 17: 39-40.

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