神经药理学报

神经药理学报››2014,Vol. 4››Issue (1): 17-19.

• 研究论文 •上一篇下一篇

和营安心方对过氧化氢损伤大鼠胚胎心肌细胞的保护作用

贺 伟， 李永民， 张海霞， 朱 娟， 高 月

  1. 欧宝平台登录 ，张家口，075000，中国
  • 出版日期:2014-02-26发布日期:2014-06-06
  • 通讯作者:李永民，男，教授，博士，硕士生导师；研究方向：中药药理学；Tel：+86-0313-4029228，E-mail: liyongmin2001@sina.com
  • 作者简介:贺伟，女，助教，硕士；研究方向：中药药理学；Tel：+86-0313-4029221；E-mail：heweihebei@163.com
  • 基金资助:

    河北省教育厅高等学校欧宝娱乐-意甲尤文图斯亚洲区域合作伙伴 课题（No. 2010215）

Protective Effects of He Ying An Xin Fang on Free Radical Injury Induced by H2O2in Myocardium Rats

HE Wei, LI Yong-min, ZHANG Hai-xia, ZHU Juan, GAO Yue

  1. Hebei North University, Zhangjiakou, 075000, China
  • Online:2014-02-26Published:2014-06-06
  • Contact:李永民，男，教授，博士，硕士生导师；研究方向：中药药理学；Tel：+86-0313-4029228，E-mail: liyongmin2001@sina.com
  • About author:贺伟，女，助教，硕士；研究方向：中药药理学；Tel：+86-0313-4029221；E-mail：heweihebei@163.com
  • Supported by:

    河北省教育厅高等学校欧宝娱乐-意甲尤文图斯亚洲区域合作伙伴 课题（No. 2010215）

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摘要：目的：研究和营安心方对过氧化氢（hydrogen peroxide，H2O2）所致大鼠胚胎心肌细胞损伤的保护作用。方法：提取和营安心方，采用H2O2建立大鼠胚胎心肌细胞氧化应激损伤模型。实验分为5组，分别为：正常对照组（Control）、过氧化氢（200μmol·L-1）模型组（Model）、和营安心方（500，250，125mg·kg-1）三个剂量组。通过四甲基偶氮唑盐（3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl tetrazolium bromide，MTT）代谢率检测心肌细胞的细胞活性，并检测细胞培养液中乳酸脱氢酶（lactate dehydrogenase，LDH）及丙二醛（malondialdehyde，MDA）的含量。结果：H2O2组细胞活性显著性降低（P＜0.01），LDH及MDA含量显著性升高（P＜0.01）；和营安心方可显著提高H2O2损伤的心肌细胞活性，减少MDA 含量（P＜0.05），降低LDH活性（P＜0.05）。结论：和营安心方对H2O2损伤的心肌细胞具有明显的保护作用，其机制可能与抑制脂质过氧化有关。

关键词:和营安心方,过氧化氢,心肌细胞,氧化应激

Abstract:Objective:To investigate the protective effects and mechanisms of he ying an xin fangon myocardium injury caused by H2O2.Methods:Hydrogen peroxide (H2O2) was adopted to establish the rat embryonic cardiomyocytes oxidative stress insult model , and the experiment was divided into 5 groups: cardiomyocytes were incubated by H2O2(200 μmol·L-1) for 2h as model group, the non- H2O2as control group, and the extraction of he ying an xin fang (500, 250, 125 mg·kg-1) as therapeutic groups.
The cell viabilityby 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide (MTT), content of malondialdehyde (MDA) and activity of lactate dehydrogenase (LDH) were measured.Resulds:Cell viabilityby MTT: compared with control group, cell Viabilityby MTT was decreased significantly in H2O2group (P< 0.01), while he ying an xin fang had no significant effect on cardiac myocytes of cell viability. Content of MDA: compared with that of H2O2group, the content of MDA was decreased in he ying an xin fang groups significantly (P< 0.05). Content of LDH: compared with that of control group, the content of LDH was decreased in he ying an xin fang groups significantly (P< 0.05).Conclusion:He
ying an xin fang has significant protective effects on free radical injury induced by H2O2in myocardium rats, and it may be related to the inhibition of lipid peroxidation.

Key words:He ying an xin fang,hydrogen peroxide (H2O2),myocardium,oxidative
stress

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