<strong>大黄酚对小鼠脑缺血再灌注引起肝损伤的保护作用</strong>

神经药理学报››2014,Vol. 4››Issue (4): 1-9.

• 研究论文 •下一篇

大黄酚对小鼠脑缺血再灌注引起肝损伤的保护作用

赵薇, 李方江, 王树

1. 欧宝平台登录药学系，张家口，075000，中国
2. 欧宝平台登录第一附属医院心血管内科，张家口，075000，中国

出版日期:2014-08-26发布日期:2015-01-20
通讯作者:王树，男，副教授，硕士生导师；研究方向：心脑血管损伤研究；Tel: 86-313-4029318， E-mail:wangshu388@163.com
作者简介:赵薇，女，硕士研究生；研究方向：心脑血管损伤研究；E-mail:670734978@qq.com
基金资助:

河北省科技支撑计划项目(No.07276166), 欧宝平台登录自然欧宝娱乐-意甲尤文图斯亚洲区域合作伙伴项目(No. 2010022)

Protective Effects of Chrysophanol on Liver Injury Induced by Cerebral Ischemia-Reperfusion in Mice

ZHAO Wei, LI Fang-jiang, WANG Shu

1. Department of Pharmacy, Hebei North University, Zhangjiakou, 075000, Hebei, China
2. Department of Cardiovascular Medicine, The First Affiliated Hospital, Hebei North University, Zhangjiakou, 075000, Hebei, China

Online:2014-08-26Published:2015-01-20
Contact:王树，男，副教授，硕士生导师；研究方向：心脑血管损伤研究；Tel: 86-313-4029318， E-mail:wangshu388@163.com
About author:赵薇，女，硕士研究生；研究方向：心脑血管损伤研究；E-mail:670734978@qq.com
Supported by:

河北省科技支撑计划项目(No.07276166), 欧宝平台登录自然欧宝娱乐-意甲尤文图斯亚洲区域合作伙伴项目(No. 2010022)

摘要/Abstract

摘要：目的：研究大黄酚（chrysophanol, Chry）对脑缺血再灌注引起肝损伤的保护作用及初步分子机制。方法：采用改良的Himori法建立小鼠脑缺血再灌注模型，随机将实验小鼠分为空白对照组、假手术组、模型组、大黄酚组(10.0, 1.0, 0.1 mg·kg-1)。采用生化方法检测小鼠血浆谷丙转氨酶（alanine aminotransferase, ALT）、谷草转氨酶（aspartate transaminase, AST）、超氧化物歧化酶（superoxide dismutase, SOD）、丙二醛（malondialdehyde, MDA）的变化；肝脏组织中SOD和MDA的变化；免疫组化法检测Caspase3的表达及原位杂交法测定caspase-3 mRNA的表达。结果：空白对照组和假手术组各项指标均无明显变化；模型组血清中ALT和AST含量增高，SOD活力减低，MDA含量增高，肝脏组织中SOD活力减低，MDA含量增高，Caspase-3及Caspase-3 mRNA表达明显增多；大黄酚组(10.0, 1.0, 0.1 mg·kg-1)血清中ALT和AST含量显著降低，SOD活力增高，MDA含量减低，肝脏组织中SOD活力增高，MDA含量减低，Caspase-3及caspase-3mRNA表达显著减少。结论：脑缺血再灌注可继发肝脏损伤，大黄酚可保护脑缺血再灌注引起的肝损伤，其机制可能是减少Caspase-3及caspase-3mRNA表达，抑制细胞凋亡，增强机体的抗氧化应激能力。

关键词:大黄酚,脑缺血再灌注,肝损伤,Caspase-3,氧化应激

Abstract:Objective: To research the protective effects of chrysophanol(Chry) on liver injury induced by cerebral ischemia-reperfusion in mice and its molecular preliminary mechanism.Methods: By improved Himori method, Cerebral ischemia-reperfusion injury model of mice was produced in conscious mice by temporarily obstructing bilateral common carotid arteries. The mice were randomly divided into six groups: control group, sham-operated group, model group, chrysophanol group (10.0, 1.0, 0.1mg·kg-1). The alanine aminotransferase(ALT), aspartate transaminase(AST), superoxide dismutase(SOD) and malondialdehyde(MDA) in the plasma and the SOD and MDA in the liver were measured with biochemical methods in mice. The expression of Caspase3 was determined by immunohistochemistry, and the expression of Caspase3 mRNA was detected using in situ hybridization assay.Results: There are no obvious pathological changes in various indicators between control group and sham-operated group. For mice of modle group, the concentration of ALT, AST, MDA increased and the activity of SOD decreaed in plasma; the concentration of MDA increased and the activity of SOD decreaed in liver; the expression of Caspase3 and Caspase3 mRNA strengthened in liver. For mice of chrysophanol group, chrysophanol(10.0, 1.0, 0.1 mg·kg-1) could decrease the concentration of ALT, AST, MDA and increase the activity of SOD in plasma; chrysophanol(10.0, 1.0, 0.1 mg·kg-1) could decrease the concentration of MDA and increase the activity of SOD in liver; and chrysophanol(10.0, 1.0, 0.1 mg·kg-1) could attenuate the expression of Caspase3 and Caspase3 mRNA in liver.Conclusions: Cerebral ischemia-reperfusion may cause liver damage secondarily. Chrysophanol showed the protective effects on injury liver in mice with cerebral-schemia reperfusion injury, perhaps the mechanism is involved in attenuating the expression of Caspase3 and Caspase3 mRNA, then inhibiting the cell apoptosis, enhancing the ability of the antioxidative stress.

Key words:chrysophanol,cerebral ischemia-reperfusion,liver injury,caspase-3,oxidative stress

中图分类号:

R965

赵薇, 李方江, 王树.大黄酚对小鼠脑缺血再灌注引起肝损伤的保护作用[J]. 神经药理学报, 2014, 4(4): 1-9.

ZHAO Wei, LI Fang-jiang, WANG Shu.Protective Effects of Chrysophanol on Liver Injury Induced by Cerebral Ischemia-Reperfusion in Mice[J]. Acta Neuropharmacologica, 2014, 4(4): 1-9.

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大黄酚对小鼠脑缺血再灌注引起肝损伤的保护作用

Protective Effects of Chrysophanol on Liver Injury Induced by Cerebral Ischemia-Reperfusion in Mice

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