神经药理学报

神经药理学报››2014,Vol. 4››Issue (6): 17-20.

• 实验方法学 •上一篇下一篇

斑马鱼—一种新型电惊厥动物模型

岳旺1，张继国2，杨杰1，周涛1，张丹参3，孙芳1，徐权汉1，张述智1，李晓玲4

  1. 1.青岛中仁药业有限公司，青岛，266329，中国
    2.泰山医学院，泰安，271016，中国
    3.河北科技大学，石家庄，050018，中国
    4.青岛市立医院，青岛，266011，中国
  • 出版日期:2014-12-26发布日期:2015-01-20
  • 通讯作者:李晓玲,博士，研究方向：神经系统疾病；Tel/Fax:+86-0532-82827191，Email:libankang1203@126.com
  • 作者简介:岳旺,教授，研究方向：神经药理。Tel/Fax:0532-85212368，Email:yuew777@aliyun.com

Zebrafish---A new animal model for studying electrical stimulation-induced convulsion

YUE Wang, ZHANG Ji-guo, YANG Jie, ZHOU Tao, ZHANG Dan-shen, SUN Fang, XUN Quan-han, ZHANG Shu-zhi, LI Xiao-ling

  1. 1.Qingdao Zhong Ren Pharmaceutical Limited Liability Company, Qingdao, 266329, China
    2.Taishan Medical College, Taian, 271016, China
    3. Hebei University of Science and Technology, Shijiazhuang, 050018, China
    4. Qingdao Municipal Hospital, Qingdao, 266011, China
  • Online:2014-12-26Published:2015-01-20
  • Contact:李晓玲,博士，研究方向：神经系统疾病；Tel/Fax:+86-0532-82827191，Email:libankang1203@126.com
  • About author:岳旺,教授，研究方向：神经药理。Tel/Fax:0532-85212368，Email:yuew777@aliyun.com

PDF (PC)

986

摘要：目的: 用斑马鱼建立一种电惊厥动物模型。方法: 50μA电流刺激斑马鱼，观察其惊搐反应； 2000μA电流刺激斑马鱼，观察其惊厥反应。地西泮、咪达唑仑、苯巴比妥、苯妥英、丙戊酸、琥珀酸预处理斑马鱼后，分别给予50μA和2000μA电流刺激斑马鱼1秒，观察药物的抗惊搐及惊厥作用。结果:50μA电流刺激1秒可使斑马鱼发生惊搐反应；2000μA刺激斑马鱼可发生类似小鼠最大电休克的惊厥反应。上述抗惊厥药物均可抑制电刺激所致的惊搐反应与惊厥反应(P＜0.05,P＜0.01)。结论: 斑马鱼可作为一种新型电惊厥模型，对于神经药理学研究和抗癫痫药筛选有意义。

关键词:斑马鱼,惊厥,电刺激,癫痫,惊搐

Abstract:Objective：To examine the validity of a new convulsion animal model using electrical stimulation in adult zebrafish.Methods: Adult zebrafish were randomly divided into subgroups (n=10 per group) and exposed to electric current (either 50 μA or 2000μA) for 1 second. In order to investigate the effects of antiepileptic drugs (AEDs) on such animal model, adult zebrafish were pretreated with one of the following AEDs: diazepam, midazolam, phenobarbital, phenytoin, valproate and succinate, respectively, prior to delivering electrical stimulation.Results: The behavior profile during weak electrical stimulation (50μA, 1s) was characterized by scared twitch reaction, including forward channeling, jumping, swinging and circle swimming. The behavior profile during strong electrical stimulation (2000μA, 1s) was characterized by convulsive reaction, being tonic, motionless, followed by rollover，coma or even death, similar to the phenotype seen in mice convulsion model using maximal electrical shocks. All the above-mentioned AEDs significantly suppressed scared twitch reaction and convulsion behavior, caused by electrical stimulation (P＜0.05 and P＜0.01 respectively).Conclusion: This is the first report on electrical stimulation-induced convulsion in adult zebrafish. Our study provides behavioral evidence in support of adult zebrafish as a valid animal model for neuropharmacological research and potential method for antiepileptics screening.

Key words:zebrafish,convulsion,electrical stimulation,epilepsy,scared twitch reaction

中图分类号:

Baidu
map