神经药理学报››2016,Vol. 6››Issue (4): 1-12.DOI:10.3969/j.issn.2095-1396.2016.04.001
• 研究论文 •下一篇
王晓茹,杜云广,王书华,安芳
出版日期:
2016-08-26发布日期:
2016-08-31通讯作者:
安芳,男,教授,硕士生导师;研究方向:中药有效成分的提取及药理活性研究;Tel:+86-0313-4029558,E-mail:wangshyh@126.com作者简介:
王晓茹,女,硕士研究生;研究方向:中药有效成分的提取及药理活性研究;Tel:+86-0313-4029558,Email:wangxrgf@126.com基金资助:
欧宝平台登录 重大项目(No.ZD1314),河北省研究生创新资助项目(No.2015-203,No.2016-288)
WANG Xiao-ru,DU Yun-guang,WANG Shu-hua,AN Fang
Online:
2016-08-26Published:
2016-08-31Contact:
安芳,男,教授,硕士生导师;研究方向:中药有效成分的提取及药理活性研究;Tel:+86-0313-4029558,E-mail:wangshyh@126.comAbout author:
王晓茹,女,硕士研究生;研究方向:中药有效成分的提取及药理活性研究;Tel:+86-0313-4029558,Email:wangxrgf@126.comSupported by:
欧宝平台登录 重大项目(No.ZD1314),河北省研究生创新资助项目(No.2015-203,No.2016-288)
摘要:目的:探讨荭草苷和牡荆苷对人红细胞氧化损伤的保护作用及其构效关系。方法:建立过氧化氢(hydrogen peroxide,H 2 O 2 )氧化损伤人红细胞的模型,实验随机分为正常组,H 2 O 2 氧化损伤组,荭草苷和牡荆苷(终浓度 10、20、40 μg·mL -1 )不同剂量组,20 μg·mL -1 维生素 C(vitamin C,VC)阳性对照组。研究比较荭草苷和牡荆苷各剂量组对 1,1- 二苯基 -2- 苦肼基(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基的清除率、氧化损伤红细胞的溶血率、活性氧(reactive oxygen species,ROS)、抗氧化酶系及 ATP 酶活性、丙二醛(malonaldehyde,MDA)、高铁血红蛋白(methemoglobin,MetHb)及巯基(sulfhydryl,-SH)含量的影响,扫描电镜观察红细胞表面形态。结果:荭草苷和牡荆苷对 DPPH 自由基都具有一定的清除能力,均能有效降低红细胞溶血率,减少 ROS、MDA 及 MetHb 含量,均能不同程度保护抗氧化酶、ATP 酶活性及增加 -SH 含量(P<0.05),显著改善红细胞膜的形态,其中 10 μg·mL -1 和 20 μg·mL -1 的荭草苷的作用效果优于相同剂量的牡荆苷,而40 μg·mL -1 的荭草苷和牡荆苷对红细胞具有相同的保护作用,且与 VC 作用相当。结论:荭草苷和牡荆苷对H 2 O 2 致氧化损伤的人红细胞具有保护作用,可能是通过对抗氧自由基,抑制脂质过氧化,保护细胞内抗氧化酶活性和细胞膜蛋白上的 -SH 来实现的,且荭草苷的保护作用效果优于牡荆苷。
王晓茹,杜云广,王书华,安芳.荭草苷和牡荆苷对H2O2氧化损伤人红细胞的保护作用[J]. 神经药理学报, 2016, 6(4): 1-12.
WANG Xiao-ru,DU Yun-guang,WANG Shu-hua,AN Fang.Protective Effects of Orientin and Vitexin on H2O2 -Induced Oxidative Stress in Human Erythrocytes[J]. Acta Neuropharmacologica, 2016, 6(4): 1-12.
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