<strong>莫诺苷对局灶性脑缺血再灌注大鼠海马CyclinD1及Cdk6的影响</strong>

神经药理学报››2013,Vol. 3››Issue (4): 1-7.

• 研究论文 •下一篇

莫诺苷对局灶性脑缺血再灌注大鼠海马CyclinD1及Cdk6的影响

刘婷婷，孙芳玲，艾厚喜，张丽，王文

首都医科大学宣武医院药物研究室，教育部神经变性病学重点实验室，北京市老年病医疗研究中心，北京，100053，中国

出版日期:2013-08-26发布日期:2014-06-27
通讯作者:王文，男，教授，博士，研究生导师；研究方向：神经药理，中药药理, E-mail：lzwwang@163.com
作者简介:刘婷婷，女，硕士研究生；研究方向：神经药理，中药药理；E-mail：liuting_416@126.com
基金资助:

1.“重大新药创制”科技重大专项（2012ZX09102-201-016）；2.国家自然科学基金（81173575）；3.北京市自然科学基金（7062031）；4.北京市教委科技创新平台项目（111219）；5.2011年北京市卫生系统高层次卫生技术人才培养计划项目（2011-3-097）；6.第52批中国博士后基金（2012M520330）；7.北京市博士后科研活动经费（106455）

Effects of Morroniside on the expression of CyclinD1 and Cdk6 in Hippocampus of Rats with Focal Cerebral Ischemia/Reperfusion Injury

LIU Ting-ting, SUN Fang-ling, AI Hou-xi, ZHANG Li, WANG Wen

Department of Pharmacology, Xuanwu Hospital of Capital Medical University, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing Geriatric Medical Research Center, Beijing 100053, China

Online:2013-08-26Published:2014-06-27
Contact:王文，男，教授，博士，研究生导师；研究方向：神经药理，中药药理； E-mail：lzwwang@163.com
About author:刘婷婷，女，硕士研究生；研究方向：神经药理，中药药理；E-mail：liuting_416@126.com
Supported by:

1.“重大新药创制”科技重大专项（2012ZX09102-201-016）；2.国家自然科学基金（81173575）；3.北京市自然科学基金（7062031）；4.北京市教委科技创新平台项目（111219）；5.2011年北京市卫生系统高层次卫生技术人才培养计划项目（2011-3-097）；6.第52批中国博士后基金（2012M520330）；7.北京市博士后科研活动经费（106455）

摘要/Abstract

摘要：目的:观察莫诺苷对局灶性脑缺血再灌注大鼠细胞周期蛋白的影响。方法:用线栓法制备大鼠局灶性脑缺血再灌注模型。将15只Sprague-Dawley大鼠随机分为假手术组，模型组，莫诺苷低、中、高剂量组(30mg·kg-1、90 mg·kg-1、270 mg·kg-1)。利用免疫组化方法检测大鼠患侧海马CyclinD1、Cdk6表达。结果:与假手术组相比，模型组CyclinD1及Cdk6表达显著增加；但给予莫诺苷治疗后，与模型组相比，莫诺苷中、高剂量能显著降低大鼠CyclinD1及Cdk6的表达。结论:莫诺苷能通过降低脑缺血再灌注后CyclinD1及Cdk6的表达而发挥神经保护作用。

关键词:莫诺苷,缺血再灌注,细胞周期蛋白,神经保护

Abstract:Objective: To explore the effects of morroniside on key cell-cycle regulator proteins in a rat model of focal cerebral ischemia-reperfusion.Methods: After the model of middle cerebral artery occlusion (MCAO) was established, 15 Sprague-Dawley rats were randomly assigned into sham group, model group, morroniside low dose (30 mg·kg-1) group, morroniside middle dose (90 mg·kg-1) group and morroniside high dose (270 mg·kg-1) group. Immunohistochemistry staining was used to measure the expression of CyclinD1 and Cdk6 in ischemic ipsilateral hippocampus.Results:Compared with sham group, the expression of CyclinD1 and Cdk6 were significantly increased in vehicle-treated ischemic-reperfusion rats. However, the expression of CyclinD1 and Cdk6 was significantly reduced by treatment of morroniside at doses of 90 mg·kg-1 and 270 mg·kg-1 after ischemia-reperfusion.Conclusion: Morroniside might play a role of neuroprotection by downregulating the expression of CyclinD1 and Cdk6 after cerebral ischemia-reperfusion.

Key words:morroniside,cerebral ischemia/reperfusion,cell-cycle regulator,neuroprotective

中图分类号:

R743.3

刘婷婷, 孙芳玲, 艾厚喜, 张丽, 王文.莫诺苷对局灶性脑缺血再灌注大鼠海马CyclinD1及Cdk6的影响[J]. 神经药理学报, 2013, 3(4): 1-7.

LIU Ting-ting, SUN Fang-ling, AI Hou-xi, ZHANG Li, WANG Wen.Effects of Morroniside on the expression of CyclinD1 and Cdk6 in Hippocampus of Rats with Focal Cerebral Ischemia/Reperfusion Injury[J]. ACTA NEUROPHARMACOLOGICA, 2013, 3(4): 1-7.

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莫诺苷对局灶性脑缺血再灌注大鼠海马CyclinD1及Cdk6的影响

Effects of Morroniside on the expression of CyclinD1 and Cdk6 in Hippocampus of Rats with Focal Cerebral Ischemia/Reperfusion Injury

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