<strong>低浓度DMSO 对SH-SY5Y 细胞存活率和氨基酸代谢的影响</strong>

神经药理学报››2015,Vol. 5››Issue (4): 8-13.

低浓度DMSO 对SH-SY5Y 细胞存活率和氨基酸代谢的影响

李双，王小琴，郭春燕，邹玉安

1 欧宝平台登录，张家口，075000，中国
2 欧宝平台登录第一附属医院，张家口，075000，中国

出版日期:2015-08-26发布日期:2016-03-03
通讯作者:郭春燕，女，教授，博士，研究生导师；研究方向：中药药效组分，中药组分对神经损伤保护作用；Email：guochy0311@163.com
作者简介:李双，2013级药学本科
基金资助:

河北省教育厅重大项目（项目编号:ZD2014075）

Effect of Low Level of DMSO on Cell Survival Rate and Amino Acid Metabolism in SH-SY5Y Cells

LI Shuang，WANG Xiao-qin，GUO Chun-yan，ZOU Yu-an

1. Hebei North University, Zhangjiakou, 075000, China
2. The First Affiliated Hospital of Hebei North, Zhangjiakou, 075000, China

Online:2015-08-26Published:2016-03-03
Contact:郭春燕，女，教授，博士，研究生导师；研究方向：中药药效组分，中药组分对神经损伤保护作用；Email：guochy0311@163.com
About author:李双，2013级药学本科
Supported by:

河北省教育厅重大项目（项目编号:ZD2014075）

摘要/Abstract

摘要：目的：研究二甲基亚砜（dimethyl sulfoxide，DMSO）对SH-SY5Y细胞存活率和氨基酸代谢的影响。方法：将细胞分为正常组和DMSO组。0.0125%浓度的DMSO作用SH-SY5Y细胞不同时间（24，48 h）后，利用MTT法测定细胞存活率；高效液相色谱法测定细胞中氨基酸的含量。结果：与正常组相比，在0.0125% DMSO作用下，细胞活力和形态无显著差异，但细胞内氨基酸含量变化有显著差异（P<0.05）。其中作用24 h，细胞中有7种氨基酸：谷氨酸（glutamic acid，Glu）、谷氨酰胺（glutamine，Gln）、甘氨酸（glycine，Gly）、脯氨酸（proline，Pro）、苯丙氨酸（phenylalanine，Phe）、异亮氨酸（isoleucine，Ile）、亮氨酸（leucine，Leu）含量有显著变化（P<0.05）；作用48 h，细胞中有12种氨基酸：谷氨酸、谷氨酰胺、甘氨酸、脯氨酸、苯丙氨酸、异亮氨酸、亮氨酸、丝氨酸（serine，Ser）、组氨酸（histidine，His）、赖氨酸（lysine，Lys）、丙氨酸（alanine，Ala）、色氨酸（tryptophan，Trp）含量有显著变化（P<0.01）。结论：细胞活力无显著差异时，细胞氨基酸代谢已有明显变化，氨基酸代谢异常可能为细胞损伤早期的一种特征。

关键词:氨基酸,高效液相色谱,SH-SY5Y细胞,二甲基亚砜

Abstract:Objective: The main objective of this article is to estimating the effect of dimethyl sulfoxide (DMSO) on livability and amino acid metabolism of SH-SY5Y cells.Methods: SH-SY5Y cells were divided into normal group and DMSO group. The DMSO group was exposed to 0.0125% DMSO in different time (24, 48 h) to investigate amino acid transform which was associated with cell damage.SH-SY5Y cell livability was determined by MTT as well as amino acids content was determined by high performance liquid chromatography.Results: Compared with the normal group, DMSO group`s 7 amino acids (glutamic acid, glutamine, glycine, proline, phenylalanine, isoleucine, leucine) decreased significantly after SH-SY5Y cells were treated with DMSO for 24 h (P<0.05),and 12 amino acids (glutamic acid, glutamine, glycine, proline, phenylalanine, isoleucine, leucine, serine, histidine, lysine, alanine, tryptophan) decreased significantly after treated with DMSO for 48 h (P<0.01). But there was no significant transform in cell vitality and morphological specificity.Conclusions: Although there was no obvious change in cell vitality and morphological specificity, but amino acid concentrations had significant transform. This finding suggests that abnormal amino acid metabolism may be a potential early and causal feature of cell damage.

Key words:amino acid,high performance liquid chromatography,SH-SY5Y cells,dimethyl sulfoxide

李双, 王小琴, 郭春燕, 邹玉安.低浓度DMSO 对SH-SY5Y 细胞存活率和氨基酸代谢的影响[J]. 神经药理学报, 2015, 5(4): 8-13.

LI Shuang, WANG Xiao-qin, GUO Chun-yan, ZOU Yu-an.Effect of Low Level of DMSO on Cell Survival Rate and Amino Acid Metabolism in SH-SY5Y Cells[J]. ACTA NEUROPHARMACOLOGICA, 2015, 5(4): 8-13.

参考文献

[1] Rajarshi Ral, Murali Krishna Mamidi, Anjan Kumar Das, et al. Diverse effects of dimethyl sulfoxide (DMSO)on the differentiation potential of human embryonic stem cells[J].Arch Toxicol, 2012, 86(4):651–661.

[2] T Delgado-Goñi, J Martín-Sitjar, R V Simões, et al. Dimethyl sulfoxide (DMSO) as a potential contrast agent for brain tumors[J]. NMR Biomed, 2013, 26(2): 173–184.

[3] Francesco Tamagnini, Sarah Scullion, Jonathan T Brown, et al. Low concentrations of the solvent dimethyl sulphoxide alter intrinsic excitability properties of cortical and hippocampal pyramidal cells[J]. PLOS ONE, 2014, 9(3):1-12.

[4] Yanan Guo, Tino Wolter, Tomáš Kuba?, et al. Molecular dynamics investigation of gluazo, a photo-switchable ligand for the glutamate receptor GluK2[J].PLOS ONE, 2015, 10(8):1-17.

[5] Hitoshi Ishibashi, Junya Yamaguchi, Yoshihisa Nakahata, et al. Dynamic regulation of glycine–GABA co-transmission at spinal inhibitory synapses by neuronal glutamate Transporter[J]. J Physiology, 2013, 591(16): 3821-3832.

[6] Angela T S Wyse, Carlos Alexandre Netto. Behavioral and neurochemical effects of proline[J]. Metab Brain Dis, 2011, 26(3):159–172.

[7] Xiao-dong Huang, Zhao-hui Lu, Zhong-wei Lv, et al. The Fas/Fas ligand death receptor pathway contributes to phenylalanine-induced apoptosis in cortical neurons[J]. PLOS ONE, 2013, 8(8):1-7.

[8] Thais T Zampieri, João A B Pedroso1, Isadora C Furigo, et al. Oral leucine supplementation is sensed by the brain but neither reduces food intake nor induces an anorectic pattern of gene expression in the hypothalamus[J].PLOS ONE, 2013, 8(12):1-13.

[9] Lasse K Bak, Maja L Johansen, Arne Schousboe, et al. Valine but not leucine or isoleucine supports neurotransmitter glutamate synthesis during synaptic activity in cultured cerebellar neurons[J]. J Neuroscience Research, 2012, 90(9): 1768–1775.

[10] Rachael Anne Dunlop, Paul Alan Cox, Sandra Anne Banack, et al. The non-protein amino acid BMAA is misincorporated into human proteins in place of L-serine causing protein misfolding and aggregation[J].PLOS ONE, 2013, 8(9):1-8.

[11] Jaime de Juan-Sanz, Enrique Nunez, Beatriz Lopez-Corcuera, et al. Constitutive endocytosis and turnover of the neuronal glycine transporter GlyT2 is dependent on ubiquitination of a c-terminal lysine cluster[J].PLoS One, 2013, 8(3):1-13.

[12] Natalia Pozdnyakova, Marina Dudarenko, Ludmila Yatsenko, et al. Perinatal hypoxia: different effects of the inhibitors of GABA transporters GAT1 and GAT3 on the initial velocity of [3H]GABA uptake by cortical, hippocampal, and thalamic nerve terminals[J].Croat Med, 2014, 55(3):250-258.

[13] F D Zepf, M Landgraf, C S Biskup, et al. No effect of acute tryptophan depletion on verbal declarative memory in young persons with ADHD[J].Acta Psychiatr Scand, 2013, 128(2):133–141.

[14] John D Fernstrom. Effects and side effects associated with the non-nutritional use of tryptophan by humans1-3[J].American Society for Nutrition, 2012, 142(12): 2236-2244.

低浓度DMSO 对SH-SY5Y 细胞存活率和氨基酸代谢的影响

Effect of Low Level of DMSO on Cell Survival Rate and Amino Acid Metabolism in SH-SY5Y Cells

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