原人参三醇组皂苷和人参皂苷-Re对MPP<sup>+</sup>诱导的SH-SY5Y细胞损伤的保护作用

神经药理学报››2011,Vol. 1››Issue (5): 1-7.

• 研究论文 •下一篇

原人参三醇组皂苷和人参皂苷-Re对MPP⁺诱导的SH-SY5Y细胞损伤的保护作用

李东薇，窦德强

辽宁中医药大学药学院，大连，116620，中国

出版日期:2011-10-26发布日期:2013-03-24
通讯作者:窦德强，男，教授，博士研究生导师；Tel：+86-0411-87406497，E-mail：doudeqiang7003@yahoo.com.cn
作者简介:李东薇，女，硕士研究生；研究方向：中药药效物质基础；E-mail：wei-5599@163.com

Protective Effect of PPT and Ginsenoside-Re in MPP⁺-treated SH-SY5Y Cells

LI Dong-wei，DOU De-qiang

College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, 116620, China

Online:2011-10-26Published:2013-03-24
Contact:窦德强，男，教授，博士研究生导师；Tel：+86-0411-87406497，E-mail：doudeqiang7003@yahoo.com.cn
About author:李东薇，女，硕士研究生；研究方向：中药药效物质基础；E-mail：wei-5599@163.com

摘要/Abstract

摘要：目的：探讨原人参三醇组皂苷(protopanaxatriol group，PPT)和人参皂苷-Re对1-甲基-4-苯基-吡啶阳离子(1-methyl-4-phenylpyridinium ion, MPP⁺）诱导的人多巴胺能神经母细胞瘤系SH-SY5Y细胞损伤的保护作用。方法：用不同浓度的PPT和人参皂苷-Re预处理细胞，以及不同体积分数的PPT和人参皂苷-Re含药血清预处理细胞，采用噻唑蓝（methylthiazolyldiphenyl-tetrazolium bromide，MTT）法检测细胞活力，计算细胞存活率，分别比较各组间的差异。结果：5 µmol·L^-1和10 µmol·L^-1的PPT和人参皂苷-Re可以增加细胞存活率（与模型组比较,P<0.05），体积分数10%的PPT和人参皂苷-Re含药血清能显著增加细胞存活率（与模型组比较，P<0.001）。结论：PPT和人参皂苷-Re对MPP⁺诱导的SH-SY5Y细胞损伤有显著的保护作用，具有潜在的抗帕金森病作用。

关键词:原人参三醇组皂苷,人参皂苷-Re,SH-SY5Y细胞,帕金森病,神经保护

Abstract:Objective:To investigate the protective effect of PPT (protopanaxatriol group) and ginsenoside-Re on 1-methyl-4-phenylpyridinium ion (MPP+)-induced damage in SH-SY5Y（human dopaminergic neuroblastoma cell line）cells.Methods:SH-SY5Y cells were treated with different concentrations of PPT, ginsenoside-Re or MPP+ containing serum. MTT colorimetry was used to test the viability of the cells and the survival rates were calculated and analyzed.Results:Cells treated by either 5 µmol•L-1, 10 µmol•L-1 PPT or ginsenoside-Reshowed significantly higher viability as compared to control group (P<0.05). In addition, 10% PPT treated or ginsenoside-Remedicated serum treated groups showed similar protective effect.Conclusion:PPT and ginsenoside-Re have neuroprotective effects in a cell model of Parkinson’s disease MPP⁺-induced SH-SY5Y cell death, suggesting that both PPT and ginsenoside-Re may have anti-PD effect.

Key words:protopanaxatriol group,ginsenoside-Re,SH-SY5Y cells,Parkinson&,rsquo,s disease,,neuroprotective effects

中图分类号:

R965

李东薇, 窦德强. 原人参三醇组皂苷和人参皂苷-Re对MPP⁺诱导的SH-SY5Y细胞损伤的保护作用[J]. 神经药理学报, 2011, 1(5): 1-7.

LI Dong-wei, DOU De-qiang. Protective Effect of PPT and Ginsenoside-Re in MPP⁺-treated SH-SY5Y Cells[J]. ACTA NEUROPHARMACOLOGICA, 2011, 1(5): 1-7.

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原人参三醇组皂苷和人参皂苷-Re对MPP⁺诱导的SH-SY5Y细胞损伤的保护作用

Protective Effect of PPT and Ginsenoside-Re in MPP⁺-treated SH-SY5Y Cells

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原人参三醇组皂苷和人参皂苷-Re对MPP+诱导的SH-SY5Y细胞损伤的保护作用

Protective Effect of PPT and Ginsenoside-Re in MPP+-treated SH-SY5Y Cells

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原人参三醇组皂苷和人参皂苷-Re对MPP⁺诱导的SH-SY5Y细胞损伤的保护作用

Protective Effect of PPT and Ginsenoside-Re in MPP⁺-treated SH-SY5Y Cells