<strong>Establishment and Behavior Evaluation of Rat Chronic Unpredictable Mild Stress and Mouse Social Defeat Models</strong>

Abstract

Abstract:Objective： To study the pathogenesis of major depressive disorder （MDD），it is necessary to establish animal models of depression. There are two common chronic depressive models that are chronic unpredictable mild stress （CUMS） and social defeat （SD）. In this study，we modified these models and compared the advantages and disadvantages of the two models to establish a more stable model of depression. Methods： For CUMS model，rats were treated with randomized two stressors last for 28 days. Stressors include overnight illumination，tail pinch，wet cage，crowding cage，ice water swimming，water and food deprivation. Rats were divided into three groups of blank Control，CUMS and CUMS + Fluoxetine （10 mg·kg-1） as positive control. For SD model，social defeat stress factors include 5 to 10 min physical attack and 24 hours sensory interaction. Mice were divided into Control and SD groups. After modeling， we adopt sucrose preference，immobility time of Forced Swimming Test and social interaction ratio to study whether the model is successful. Results： There was a significant decrease of sucrose preference （69.3%±3.6%，n=27） and significant increase of immobility time of Forced Swimming Test （131.2±5.8 s，n=27） in CUMS rats，as compared with control group of rats with sucrose preference （81.0%±3.2%，n=17，P<0.05） and immobility time （105.1±8.2 s，n=17， P<0.05）. The sucrose preference （82.1%±3.9%，n=10） and immobility time of Forced Swimming
Test （108.3±9.7 s，n=10） were no significant change in CUMS + Flu group rats，as compared with control group. Mice in social defeat showed that social interaction ratio below 1 （determined by SIR<1，SIR=Duration in interaction zone of target absent phase/ Duration in interaction zone of target present phase），as compared with social interaction ratio greater than 1 in control group. Moreover，SIR of SD mice was significantly lower （69.2%±2.4%，n=16） than that of control group （78.6%±3.2%，n=9，P<0.05）. Conclusions： Based on the core criteria of sucrose preference，models of CUMS in rats and SD in mice are successfully established，whereas the social defeat model is more reproducible and consistent.

Key words:major depressive disorder,chronic unpredictable mild stress model,social defeat,sucrose preference

CLC Number:

R964

XIAO Ting, MA Tian-yang, XU Xiang-qing, WANG Ke-wei.Establishment and Behavior Evaluation of Rat Chronic Unpredictable Mild Stress and Mouse Social Defeat Models[J]. ACTA NEUROPHARMACOLOGICA, 2018, 8(1): 45-53.

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Establishment and Behavior Evaluation of Rat Chronic Unpredictable Mild Stress and Mouse Social Defeat Models

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