Comparison in Tissue Distribution and Pharmacodynamics Effects between Three Chrysophanol Formulations in Mice with Cerebral Ischemia Reperfusion

Abstract

Abstract:Objective：To compare the pharmacodynamics and the tissue distribution of chrysophanol loaded polybutylcyanoacrylate nanocapsules，chrysophanol-hydroxypropyl-β-cyclodextrin inclusion complex，chrysophanol liposomes and DMF dissolved chrysophanol in mice with cerebral ischemia reperfusion.Methods：Using the cerebral ischemia reperfusion injury model，determined the chrysophanol content in blood，brain，heart，kidney，liver，spleen，lung of mice；measured the activity of malondialdehyde (MDA)，monoamine oxidase-B (MAO-B)，nitric oxide (NO)，nitric oxide synthase (NOS)，lipofuscin (LF)，acetylcholinesterase (AChE) in brain，the content of amino acid (AA) in brain and the content of lipofuscin (LF) in heart；observed the morphology of hippocampal.Results：The order of chrysophanol concentration in mouse tissues is：blood>brain>kidney>liver>heart>spleen>lung. Compared with chrysophanol DMF，chrysophanol concentrations of chrysophanol formulations (10.0，1.0，0.1 mg·kg^-1dose groups) in brain，liver and blood tissues were significantly high (P<0.01~0.05) in a dose-dependent manner. At the same time，the aging-related enzymes’ activity and the pathological morphology in hippocampal area were improved to some extent.Conclusion：All the three chrysophanol formulations could enhance the anti-aging effects of chrysophanol in mice with cerebral ischemia reperfusion，with the possible mechanisms involving enhancement of chrysophanol content in brain，regulation of the activity of aging-related enzymes and improvement of the pathological morphology in hippocampi area.

Key words:chrysophanol,chrysophanol loaded polybutylcyanoacrylate nanocapsules,chrysophanol-hydroxypropyl-beta,-cyclodextrin inclusion complex,chrysophanol liposomes,cerebral ischemia reperfusion,tissue distribution,aging-related enzymes,tissue pathology

CLC Number:

R965
R361

LI Chao, ZHANG Li. Comparison in Tissue Distribution and Pharmacodynamics Effects between Three Chrysophanol Formulations in Mice with Cerebral Ischemia Reperfusion[J]. ACTA NEUROPHARMACOLOGICA, 2011, 1(4): 16-25.

References

1 王树, 张丹参, 张力, 等. 大黄酚对脑缺血/再灌注小鼠记忆功能的保护作用[J]. 中国老年学杂志, 2009, 29(15): 1934-1936.
2 李超, 张丹参, 赵晓倩, 等. 大黄酚三种制剂改善脑缺血/再灌注小鼠记忆功能的实验筛选研究[J]. 中国药理学通报, 2010, 26(12): 1607-1612.
3 Himori Norio, Watanabe Hiroshi, Akaike Nobuhide,et al. Cerebral ischemia model with consciousmice: involvement of NMDA receptor activation and derangement of learning and memory ability[J]. J Pharmacol Methods, 1990, 23(4): 311-327.
4 黄红娜, 张丹参, 郑晓霞, 等. 大黄酚聚氰基丙烯酸丁酯纳米囊的制备工艺及质量研究[J]. 中草药, 2010, 41(4): 547-550.
5 MeCann SK, DustingG J, Roulston C L. Early increase of Nox4 NADPHoxidase and super oxide generation following endothelin-1-induced stroke in conscious rats[J]. J Neurosci Res, 2008, 86(11): 2524-2534.
6 Terao Y, OhtaH, Oda A,et al. Macrophage inflammatory protein-3alpha plays a key role in the inflammatory cascade in rat focal cerebral ischemia[J]. Neurosci Res, 2009, 64(1): 75-82.
7 况时祥, 刘继刚, 肖燕, 等. 脑通胶囊对血管性痴呆大鼠学习记忆能力及脑组织NO, SOD含量的影响[J]. 中国中医急症, 2010, 19(8): 1368-1370.
8 张会珍, 李文丽, 马小顺, 等. 电针对拟血管性痴呆小鼠SOD和MDA的影响[J]. 中国中医基础医学杂志, 2010, 16(11): 1034-1035.
9 刘秋芳, 逯晓波, 靳翠红, 等. 碱性电解水对D-半乳糖致小鼠血液和肝, 肾,脑组织脂质过氧化作用的影响[J]. 环境与健康杂志, 2010, 27(2): 105-107.
10 张晓杰, 张静艳, 赵坤, 等. 通络救脑口服液对AD大鼠海马单胺氧化酶活性, 脂褐素含量的影响[J]. 中国老年学杂志, 2010, 30, (18): 2634-2636.
11 Tsai SK, Hung LM, Fu YT,et al. Resveratrol neuroprotective effects during focal cerebral ischemia injury via nitric oxidemechanism in rats[J]. J Vasc Surg, 2007, 46(2): 346-353.
12 熊文欣, 李文峰. 硫化氢对脑缺血再灌注损伤大鼠血清及脑组织NO和NOS的影响[J]. 现代中西医结合杂志, 2010, 19(16): 1971-1972.
13 姚五湖, 龙斌, 周日笑. 贯叶连翘提取物提高衰老小鼠学习记忆能力的研究[J]. 今日药学, 2010, 20(4): 37-39.
14 苗艳艳, 苗明三, 马霄, 等. 五味子醇提部位对反复脑缺血再灌注模型小鼠脑能量代谢的影响[J]. 中华中医药杂志, 2009, 24(9): 1207-1209.
15 刘杰, 华赟鹏, 谭敏谊, 等. 芍药苷注射液对脑缺血-再灌注沙土鼠脑组织中兴奋性氨基酸及腺苷含量的影响[J]. 中药材, 2010, 33(9): 1456-1460.
16 张新春, 吕光耀, 秦秀德. 益气活血中药对大鼠缺血-再灌注脑组织中氨基酸含量的影响[J]. 中国医药导报, 2009, 6(22): 30-32.
17 陆晖, 吴云虎, 陆艳玲, 等. 酸枣仁皂苷A对脑缺血再灌注损伤大鼠神经行为学及脑组织氨基酸含量的影响[J]. 广西中医药, 2008, 31(6): 47-50.
18 Yu J, Novgorodov SA, Chudakova D,et al. JNK3 signaling pathway activates ceramide synthase leading to mitochondrial dysfunction[J]. J Biol Chem, 2007, 282(35): 25940-25949.
19 张兴毅, 宋国林, 韩江全, 等. 全脑缺血再灌注后大鼠海马回中Bcl-2及Bax的表达[J]. 西部医学, 2009, 21(11): 1850-1852.

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