<strong>A Method of Separating and Culturing Astrocytes from Rat Cerebral Cortex</strong>

Acta Neuropharmacologica››2011,Vol. 1››Issue (6): 22-27.

A Method of Separating and Culturing Astrocytes from Rat Cerebral Cortex

FAN Yue，YAN Yong，SHANG Ya-zhen

Hebei Provincial Key Laboratory of Traditional Chinese Medicine Research and Development, Institute of Traditional Chinese Medicine, Chengde Medical College, Chengde 067000, China

Online:2011-12-26Published:2013-04-25
Contact:商亚珍，Tel：+86-0314-2290616，E-mail：shangyz1018@ yahoo.com.cn
About author:范悦，女，承德医学院2010届硕士研究生
Supported by:

河北省自然基金（No. C2009001007），河北省中医药管理局资助项目（No. 05027）

Abstract

Abstract:Objective:To establish a method for rat cerebral cortical astrocyte culture, purification and identification.Methods:The cerebral cortex cells of newborn rats (1-3 days old) were cultured. The fibroblast cells and neurons were removed through differential adhesion and passage. Glial fibrillary acidic protein (GFAP), an astrocyte specific protein, was used for identifying astrocytes by immunohistochemical method.Results:The cultured cells showed typical morphological features of astrocyles and grew well. The third generation of the cells comprised > 95% astrocytes.Conclusions:The method is a simple and reliable in vitro culture for obtaining high purity astrocytes from newborn rat cerebral cortex.

Key words:astrocytes,in vitro,immunohistochemical,glial fibrillary acidic protein

CLC Number:

R965.2

FAN Yue，YAN Yong，SHANG Ya-zhen.A Method of Separating and Culturing Astrocytes from Rat Cerebral Cortex[J]. Acta Neuropharmacologica, 2011, 1(6): 22-27.

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A Method of Separating and Culturing Astrocytes from Rat Cerebral Cortex

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