Abstract:Objective:To study antioxidant enzymes activity and lipofuscin dynamic effects of orientin and vitexin inTrollius chinensison aging mice induced by D-Galactose(D-gal) and find the time effect relationship of antioxidant.Methods:The aging mice model were induced by intraperitoneal injection of D-gal, and it was successful after 8 weeks. Divided into blank group, modelgroup, high, medium and low dose group of orientin and vitexin, Vitamin E control group. From the 9 th week, every morning each group will be given medicine. The route is oral administration, and the time is 8 weeks. Decapitated mice at the 2 th , 4 th , 6 th and 8 th week , resected brain in frozen conditions, 10% tissue homogenate were made with NS solution, then got supernatant by use of centrifugation in 8000 r, detected superoxide dismutase(SOD), catalase(CAT), glutathione peroxidase(GSH-PX) and lipofuscin(LPF).Results:Compared with model group, the activity of antioxidase in each therapy group increased slowly after 2 and 4 weeks, but not statistically significant , and after 6 and 8 weeks, orientin and vitexin significantly improved the activity of antioxidase, and significantly reduced the amount of LPF. Compared orientin with vitexin, the activity of antioxidase and the amount of LPF in the same dose group was no statistically significant after 2 and 4 weeks. The activity of antioxidase in each dose group of orientin were better than that of the vitexin of the same dose. they significantly reduced lipofuscin levels in the brain after 6 weeks and orientin was superior to vitexin at the same dose. The activity of antioxidase in medium and low dose group of orientin was better than the same vitexin, they significantly reduced lipofuscin levels in the brain and high dose group have no significant difference after 8 weeks．Compared with VE group, after 8 weeks, the activity of antioxidase in medium and low dose group of orientin and vitexin were lower than VE group, they have significant difference, meanwhile, high dose group have no significant difference.Conclusion：Orientin and Vitexin can apparently improve activity of antioxidation and reduce lipofuscin levels in brain. The activity of antioxidase became increased with the concentration increasing and reaction time prolonging. After 8 weeks, high dose group of orientin and vitexin have the same function with VE.

Key words:orientin,vitexin,D-galactose,antioxidation,brain tissue