<strong>The Effect of Polygalasaponin F on Rotenone-induced PC12 Cells and the Expression of CREB</strong>

Acta Neuropharmacologica››2013,Vol. 3››Issue (1): 12-16.

The Effect of Polygalasaponin F on Rotenone-induced PC12 Cells and the Expression of CREB

WU Miao-miao¹，YUAN Yu-he²，CHEN Nai-hong²

1. Department of Pharmacy Hebei North University，Zhangjiakou，075000，China 2. State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica，Chinese Academy of Medical Science and Peking Union Medical College，Beijing，100050，China

Online:2013-02-26Published:2014-06-27
Contact:陈乃宏，男，研究员，博士生导师；Tel.: +86-010-63165177，Fax: +86-010-63165177，E-mail: chennh@imm.ac.cn
About author:吴苗苗，女，助教，硕士；研究方向：神经药理学；Tel：+86-0313-4025966，E-mail：miaomiao2076924@126.com
Supported by:

国家自然科学基金（No.81274122、No.81102831、No.81173578），创新药物研究开发技术平台建设（No.2012ZX09301002-004、2012ZX09103101-006）

Abstract

Abstract:Objective：To investigate the effect of polygalasaponin F (PS-F) on the PC12 cells insulted by rotenone and the expression of CREB.Methods：The cell morphology was observed by inverted microscope. Caspase-3 activity was determined by caspase 3 activity assay kit. The expression of CREB was examined by luciferase reporter assay system.Results:When exposed to 4 μmol·L^-1rotenone, a lot of PC12 cells were apoptotic, and the activity of caspase 3 was increased significantly. When treated with PS-F, the damage of the cells was reduced significantly and the caspase 3 activity was decreased significantly. PS-F could increase the expression of CREB.Conclusion:PS-F protects PC12 cells against apoptosis induced by rotenone and the neuroprotective effect may be related to increasing the expression of CREB.

Key words:Parkinson&,rsquo,s diseases,rotenone,apoptosis,caspase 3,CREB

WU Miao-miao，YUAN Yu-he，CHEN Nai-hong.The Effect of Polygalasaponin F on Rotenone-induced PC12 Cells and the Expression of CREB[J]. Acta Neuropharmacologica, 2013, 3(1): 12-16.

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The Effect of Polygalasaponin F on Rotenone-induced PC12 Cells and the Expression of CREB

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