<strong>Studies on Synthesis and Structure Characterization and Biological Activity of Orientin-Zinc Complexes from Trollius Chinensis Bunge</strong>

ACTA NEUROPHARMACOLOGICA››2013,Vol. 3››Issue (2): 26-34.

Studies on Synthesis and Structure Characterization and Biological Activity of Orientin-Zinc Complexes from Trollius Chinensis Bunge

JIANG Wei，CAO Xin-xin，WANG Shu-lin，WANG Shu-hua

College of Pharmacy, Hebei North University, Zhangjiakou, 075000, China

Online:2013-04-26Published:2014-06-27
Contact:王书华，教授，硕士生导师。主要从事中药有效成分的提取及药理研究。Email：wangshyh@126.com
About author:蒋伟，欧宝平台登录 2009级药理学硕士研究生， Email: 574941084@qq.com
Supported by:

张家口市科技局资助项目（11110015D）欧宝平台登录重大项目(ZD1314)

Abstract

Abstract:

Objective: To optimize the synthetic process conditions of and study the antioxidation activity of orientin and orientin zinc complexes in vitro. Methods: Using elemental analyzer, plasma emission spectrometer, ultraviolet spectrometer, infrared spectrometer, thermogravimetric analyzer and nuclear magnetic resonance instrument, the structural characterization of orientin zinc complexes was observed, taking superoxide anion radicals, hydroxyl radicals, 1,1-Diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH)radical generation system as experimental system, to examine the antioxidant activity of orientin and orientin-zinc complexes. Results: The best coordination reaction optimum process of complexes formed by orientin and zinc acetate was shown as follows. Taking anhydrous ethanol as solvent, reaction pH was 8, reaction temperature was 65℃, and reaction time was 8 h. The orientin-zinc complexes maybe the chemical formula [Zn3(C21H15O11)2]?4H2O and chemical structural formula. Orientin and orientin-zinc complexes have clear ability of removing ·OH and ·DPPH, and the effect of orientin-zinc complexes in each dose group was more obvious than equivalent dose orientin at 1.0~8.0μg·mL-1. Conclusion: Orientin-zinc complexes is more superior than orientin on the activity of antioxidation.

Key words:trollius chinensis bunge,orientin-zinc complexes,structural characterization,antioxidation

JIANG Wei, CAO Xin-xin, WANG Shu-lin, WANG Shu-hua.Studies on Synthesis and Structure Characterization and Biological Activity of Orientin-Zinc Complexes from Trollius Chinensis Bunge[J]. ACTA NEUROPHARMACOLOGICA, 2013, 3(2): 26-34.

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Studies on Synthesis and Structure Characterization and Biological Activity of Orientin-Zinc Complexes from Trollius Chinensis Bunge

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