<strong>Screening the Optimal Combination Ratio of Active Radix Salviae Miltiorrhizae Ingredients for Their Antioxidant and Hippocampal Neurons Protection Effects Using Uniform Design-High Throughput Screening Technology</strong>

ACTA NEUROPHARMACOLOGICA››2013,Vol. 3››Issue (3): 8-14.

Screening the Optimal Combination Ratio of Active Radix Salviae Miltiorrhizae Ingredients for Their Antioxidant and Hippocampal Neurons Protection Effects Using Uniform Design-High Throughput Screening Technology

JIN Can，ZHANG Dan-shen

Department of Pharmacy，Hebei North University，Zhangjiakou，075000，China

Online:2013-06-26Published:2014-06-27
Contact:张丹参，女，教授，博士生导师；研究方向：神经药理学；Tel：+86-0313-4029556，E-mail：zhangds2011@126.com
About author:金灿，女，硕士研究生；E-mail：cking5168@163.com
Supported by:

国家自然科学基金项目（No.81274005），河北省自然科学基金项目（No.C2009001026）

Abstract

Abstract:Objective:To implement uniform design-high throughput screening (UD-HTS) technology for screening the antioxidant and hippocampal neurons protection activities of radix salviae miltiorrhizae (RSM) ingredients using multifactor and multilevel combination design.Methods: Bioactive ingredients of RSM (7 liposoluble and 7 water-soluble) were combined with different ratios through uniform design. The bioactivity of the combination samples were assessed by anti-DPPH oxidation activity method (screening) and cytological experiment (rescreening).Results:Two optimal combination samples of the antioxidant and hippocampal neurons protection, A13 and PA, were identified.Conclusion: UD-HTS technology is suitable for multifactor and multilevel screening, which can provide rapid screening of optimal combinations.

Key words:radix salviae miltiorrhizae,uniform design-high throughput screening,2,2-Di(4-tert-octylphenyl)-1-picrylhydrazyl（DPPH）,hippocampal neurons

CLC Number:

R965

JIN Can, ZHANG Dan-shen.Screening the Optimal Combination Ratio of Active Radix Salviae Miltiorrhizae Ingredients for Their Antioxidant and Hippocampal Neurons Protection Effects Using Uniform Design-High Throughput Screening Technology[J]. ACTA NEUROPHARMACOLOGICA, 2013, 3(3): 8-14.

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Screening the Optimal Combination Ratio of Active Radix Salviae Miltiorrhizae Ingredients for Their Antioxidant and Hippocampal Neurons Protection Effects Using Uniform Design-High Throughput Screening Technology

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