<strong>Effect of Propofol on the Cognitive Function and NMDAR2B Expression of CA1 in the Hippocampus of Aged Rats</strong>

Acta Neuropharmacologica››2015,Vol. 5››Issue (3): 1-5.

Effect of Propofol on the Cognitive Function and NMDAR2B Expression of CA1 in the Hippocampus of Aged Rats

QIAO Lin, ZHAO Wei, WANG Xin-sheng, XING Zhen

HeBei North University, Zhangjiakou, 075000, China

Online:2015-06-26Published:2015-07-06
Contact:邢珍，女，医学硕士，教授，硕士研究生导师；研究方向：麻醉药物与POCD
About author:乔霖，男，硕士研究生；研究方向：静脉麻醉药与POCD
Supported by:

欧宝平台登录自然欧宝娱乐-意甲尤文图斯亚洲区域合作伙伴计划项目（重大项目：120176）

Abstract

Abstract:Objective：The effect of propofol anesthesia on learning memory and NMDAR2B expression of CA1 in hippocampus were observed and the effect of propofol on postoperative cognitive dysfunction（ POCD） was discussed.Methods：Male SD aged rats were randomly divided into control group and propofol group，with 20 rats in each group. Propofol group was given 50 mg·kg-1 propofol by intraperitoneal injection. The control group was given the same dose of emulsion. The Morris water maze test was performed on 24 h after the end of anesthesia. The expression of NMDAR2B protein and gene transcription of CA1 region were detected by immunofluorescence and FISH.Results：In Morris water maze test：compared with the control group，the latent period was signifi cantly prolonged，and the crossing times of propofol group were signifi cantly decreased. In immunofluorescence detection，the expression of NMDAR2B was significantly decreased in propofol group. In FISH，compared with the control group，the expression of NMDAR2B decreased significantly.Conclusion：Propofol anesthesia can induce a short-term cognitive dysfunction in aged rats，and its mechanism may be related to the down-regulation of NMDAR2B expression in hippocampus CA1 region.

Key words:Propofo,POCD,NMDA2B receptor

CLC Number:

R338.64
R965

QIAO Lin, ZHAO Wei, WANG Xin-sheng, XING Zhen.Effect of Propofol on the Cognitive Function and NMDAR2B Expression of CA1 in the Hippocampus of Aged Rats[J]. Acta Neuropharmacologica, 2015, 5(3): 1-5.

References

[1] Lauren Pain, Marie-Josee Angst, Laurence LeGourrier, et al. Effect of a nonsedative does of propofol on memory for aversively loaded information in rats[J]. Anaesthesiology, 2002, 97(2):447-445.

[2] Jiang Peng, Ling Qiong, Liu Hong-bo, et al. Intracisternal administration of an interleukin-6 receptor antagonist attenuates surgery-induced cognitive impairment by inhibition of neuroinflammatory responses in aged rats [J].Exp Ther Med, 2015, 9(3):982-986.

[3] Zhang Yi-ying, Dong Yuan-lin, Xu Zhi-peng, et al. Propofol and magnesium attenuate isoflurane induced caspase-3 activation via inhibiting mitochondrialpermeability transition pore [J].Med Gas Res, 2012, 2(1):20.

[4] Saraswathi Subramaniyan, Vahid Hajali, Thomas Scherf, et al. Hippocampal receptor complexes paralleling LTP reinforcement in the spatial memory holeboard test in the rat[J]. Behav Brain Res, 2015, 283:162-174.

[5] Lorenz Muller, Tursonjan Tokay, Katrin Porath, et al. Enhanced NMDA receptor-dependent LTP in the epileptic CA1 area via upregulation of NR2B [J]. Neurobiology Dis, 2013, 54: 183-193.

[6] Hyungju Park, Kyung-Seok Han, Jinsoo Seo, et al. Channel-mediated astrocytic glutamate modulates hippocampal synaptic plasticity by activating postsynaptic NMDA receptors [J]. Molecular Brain, 2015, 8:7.

[7] Magdalena Sanhueza, John Lisman. The CaMKII/NMDAR complex as a molecular memory [J]. Molecular Brain, 2013, 6: 10.

[8] John Lisman, Ryohei Yasuda, Sridhar Raghavachari. Mechanisms of CaMKII action in long-term potentiation[J]. Nat Rev Neurosci, 2012, 13(3): 169-182.

[9] Anatoli Y Kabakov, John E Lisman. Catalytically dead αCaMKII K42M mutant acts as a dominant negative in the control of synaptic strength[J]. PLoSONE 2015, 10(4): e0123718.

[10] Hager A M, Gagolewicz P J, Rodier S, et al. Metaplastic up-regulatio of LTP in the rat visual cortex by monocular visual training: requirement of task mastery,hemispheric specificity, and NMDA-GluN2B involvement[J].Neuroscience, 2015, 293: 171-86.

[11] Iris B Hovens, Regien G Schoemaker, Eddy A van der Zee, et al. Thinking through postoperative cognitive dysfunction: How to bridge the gap between clinical and pre-clinical perspectives [J]. Brain Behavior Immunity, 2012, 26(7): 1169-1179.

Effect of Propofol on the Cognitive Function and NMDAR2B Expression of CA1 in the Hippocampus of Aged Rats

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