Abstract:Objective: To discuss correlation of Th1/Th2 cells in balance and PARP-1 expression in rat with cerebral ischemia/reperfusion injury.Methods: SD rats were subjected to middle cerebral artery occlusion by suture method, then they were randomly divided into the control group, sham group, model group, PJ34 group, then groups were divided into 5 subgroups according to different reperfusion time points, 6, 12, 24, 48, 72 h, according to the different time points, neurological deficits of each rat were determined infarct volume was analyzed with 2, 3, 5- triphenyltetrazolium chloride (TTC) staining, the protein and mRNA expression of PARP-1 were detected by real-time quantitative PCR and western blot; the level of INF-γ and IL-4 were determined by ELISA methods.Results: compare to sham group, the neurological scores, infarct volume, PARP-1 expression and the Th1/Th2 ratio significantly increased at different time points of reperfusion(P<0.01), and reached a peak by 24h. Compare to model group, neurological scores, infarct volume, PARP-1 expression and the Th1/Th2 ratio significantly reduced in PJ34 group(P<0.01), especially in ischemia reperfusion 24h. PARP-1 expression and the Th1/Th2 ratio had significant positive correlation(r=0.984, P<0.05).Conclusion: Th1/Th2 cells in Balance had related to PARP-1 expression in rat with cerebral ischemia/reperfusion injury.

Key words:Poly（ADP-ribose）polymerase-1(PARP-1),Th1/Th2 ratio,middle cerebral artery occlusion