<strong>Effect of Low Level of DMSO on Cell Survival Rate and Amino Acid Metabolism in SH-SY5Y Cells</strong>

ACTA NEUROPHARMACOLOGICA››2015,Vol. 5››Issue (4): 8-13.

Effect of Low Level of DMSO on Cell Survival Rate and Amino Acid Metabolism in SH-SY5Y Cells

LI Shuang，WANG Xiao-qin，GUO Chun-yan，ZOU Yu-an

1. Hebei North University, Zhangjiakou, 075000, China
2. The First Affiliated Hospital of Hebei North, Zhangjiakou, 075000, China

Online:2015-08-26Published:2016-03-03
Contact:郭春燕，女，教授，博士，研究生导师；研究方向：中药药效组分，中药组分对神经损伤保护作用；Email：guochy0311@163.com
About author:李双，2013级药学本科
Supported by:

河北省教育厅重大项目（项目编号:ZD2014075）

Abstract

Abstract:Objective: The main objective of this article is to estimating the effect of dimethyl sulfoxide (DMSO) on livability and amino acid metabolism of SH-SY5Y cells.Methods: SH-SY5Y cells were divided into normal group and DMSO group. The DMSO group was exposed to 0.0125% DMSO in different time (24, 48 h) to investigate amino acid transform which was associated with cell damage.SH-SY5Y cell livability was determined by MTT as well as amino acids content was determined by high performance liquid chromatography.Results: Compared with the normal group, DMSO group`s 7 amino acids (glutamic acid, glutamine, glycine, proline, phenylalanine, isoleucine, leucine) decreased significantly after SH-SY5Y cells were treated with DMSO for 24 h (P<0.05),and 12 amino acids (glutamic acid, glutamine, glycine, proline, phenylalanine, isoleucine, leucine, serine, histidine, lysine, alanine, tryptophan) decreased significantly after treated with DMSO for 48 h (P<0.01). But there was no significant transform in cell vitality and morphological specificity.Conclusions: Although there was no obvious change in cell vitality and morphological specificity, but amino acid concentrations had significant transform. This finding suggests that abnormal amino acid metabolism may be a potential early and causal feature of cell damage.

Key words:amino acid,high performance liquid chromatography,SH-SY5Y cells,dimethyl sulfoxide

LI Shuang, WANG Xiao-qin, GUO Chun-yan, ZOU Yu-an.Effect of Low Level of DMSO on Cell Survival Rate and Amino Acid Metabolism in SH-SY5Y Cells[J]. ACTA NEUROPHARMACOLOGICA, 2015, 5(4): 8-13.

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Effect of Low Level of DMSO on Cell Survival Rate and Amino Acid Metabolism in SH-SY5Y Cells

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