Abstract:Objective：To observe the effect of Kangnaoye on nerve regeneration related factor in rats of cerebral ischemia-reperfusion injury.Methods：150 healthy adult male Sprague Dawley rats were randomly divided into 5 groups；Kangnaoye high，middle and low dose group （24、12、6 g·kg^-1·d^-1），sham operated group and model group. Longa method was used to establish the middle cerebral artery occlusion（MACO） model. TTC staining method
was used to observe infarction volume change in rats after 24 h. Collecting specimens in 1 d、3 d、7 d and 14 d，immunohistochemical method was used to observe the change of the growth associated protein-43 （GAP-43） after cerebral ischemic and the expression of neurite outgrowth inhibitor-A （NOGO-A） protein .The nervous function deficit scores were evaluated at 2 h、1 d、3 d、7 d、14 d after reperfusion.Results：Compared with model group，the
neurological functions were improved significantly in kangnaoye groups（P<0.05）. Kangnaoye high，middle and low dose groups were significantly decreased than model group in cerebral infarction volume ratio （P<0.01）. The expression levels of GAP-43 were also significantly higher in kangnaoye high and middle dosages groups than model group，at the same time the expression of NOGO-A were lower，which has significant differences （P<0.05 or P<0.01）.Conclusion：Kangnaoye can promote neurotization and improve the motor skill recovery after cerebral infarction.

Key words:kangnaoye,cerebral ischemia-reperfusion,nerve regeneration