Abstract:Objective:To investigate the effects of chlorogenic acid on granular cell in cerebellum induced by amyloid β- protein -induced macrophage activationin vitro.Methods:The experiment was divided into blank control group, Aβ_25-35treatment group and Aβ_25-35+CHA co-processing of different time group; Blank control group: Equal amount of culture medium; Aβ_25-35treatment group: with Aβ_25-3510 μmol·L^-1; Aβ_25-35and different time of CHA treatment group; The co-cultured system of cerebellar granular cells and macrophages were cultivated for 2 days and then the 10 μmol·L^-1Aβ_25-35and 1000 μmol·L^-1CHA were added into this system for 6, 12, 24, 48, 96 h respectively. The growth phynotype of neurons and macrophages were detected by inverted phase contrast microscope; The expressions of p38MAPK, MAPKAPK-2, HSP27 protein were tested by Western blot.Results:The numbers cerebellar granule cell of chlorogenic acid protection group was increased significantly compared with Aβ_25-35injury group. CHA could inhibite increased expression of phosphorylated p38MAPK of macrophage induced by Aβ_25-35at difference time; Decreased expression of phosphorylated MAPKAPK-2 and phosphorylated HSP-27of macrophage induced by Aβ_25-35could increased after the 1000 μmol·L^-1CHA added into the medium.Conclusion:The protection of Chlorogenic acid for cerebellar granular cells induced by Aβ_25-35via inhibition the activation of p38MAPK signaling pathway, which decreased the expression of p38MAPK and increased the expression of MAPKAPK-2, HSP-27.

Key words:chlorogenic acid,A&beta,_25-35,CGC,macrophage,p38MAPK,MAPKAPK-2,HSP27