Effects of Chrysophanol Liposomes on Apoptosis of the Hippocampal Neurons in Mice after Cerebral Ischemia and Reperfusion Injury

Abstract

Abstract:Objective:To observe the influence of chrysophanol liposomes on apoptosis of hippocampal neurons in mice and to study the protective effects on cerebral ischemia and reperfusion injury and its mechanism.Methods:Cerebral ischemia and reperfusion injury was produced in conscious mice by temporarily obstructing bilateral common carotid arteries. Chrysophanol liposomes（10.0，1.0，0.1 mg· kg^-1）was injected intraperitoneally. The effects of chrysophanol liposomes were studied on the neurological function, the pathomorphology of brain tissues and the expression of apoptosisrelated protein caspase-3, Bcl-2 and Bax in hippocampal neuron using immunohistochemistry.Results： Chrysophanol liposomes could increase the expression of Bcl-2, decrease the expression of Bax and caspase-3, improve the neurological function score and decrease the pathological change in chrysophanol liposomes treated animals. The effect of chrysophanol liposomes was most obviously in group 10.0 mg· kg^-1（P<0.05）.Conclusion: Chrysophanol liposomes have a significant protective effect on hippocampal neurons after cerebral ischemia and reperfusion injury. The mechanism may be related to increasing the expression of Bcl-2, reducing caspase-3 and Bax.

Key words:chrysophanol liposomes,cerebral ischemia and reperfusion injury,hippocampal,apoptosis,caspase-3,Bcl-2,Bax

CLC Number:

R971

SONG Jin-yan, ZHANG Li, ZHAO Xiao-qian, SONG Zhi-bin. Effects of Chrysophanol Liposomes on Apoptosis of the Hippocampal Neurons in Mice after Cerebral Ischemia and Reperfusion Injury[J]. ACTA NEUROPHARMACOLOGICA, 2011, 1(2): 7-13.

References

[1] Wu Pengfei, Zhang Zui, Wang Fang, et al. Natural compounds from traditional medicinal herbs in the treatment of cerebral ischemia/reperfusion injury [J].Acta Pharmacol Sin,2010,31(12):1523-1531.

[2] 李超,张丹参,赵晓倩,等.三种大黄酚制剂改善脑缺血/再灌注小鼠记忆功能的实验筛选研究[J].中国药理学通报,2010,26(12):1607-1612.

[3] 王树,张丹参,张力,等.大黄酚对脑缺血再灌注小鼠记忆功能的保护作用[J].中国老年学杂志,2009,29(15):1934-1936.

[4] 董晓华,张丹参.大黄酚对大鼠海马齿状回突触传递长时程增强的影响[J].中国药科大学学报,2009,40(2):152-156.

[5] Himori N, Watanabe H, Akaike N, et al. Cerebral ischemia model with conscious mice: involvement of nmda receptor activation and derangement of learning and memory ability[J]. Pharmacol Meth, 1990,23(4):311-327.

[6] Julio H.Garcia, Simone Wagner, Liu Kaifeng, et al. Neurological deficit and extent of neuronal necrosis attributable to middle cerebral artery occlusion in rats[J].Stroke,1995,26(4):627-635.

[7] Siesjö BK. Pathophysiology and treatment of focal cerebral ischemia. PartI:Pathophysiology [J]. Neurosurg, 1992,77(2):169-84.

[8] 张均田.神经元-突触丢失与老年痴呆[J].神经药理学报,2011,1(1):1-15.

[9] 张丹参.几种适合药物筛选的抗氧化实验微孔板测定方法及其稳定性研究[J]. 神经药理学报,2011,1(1):45-50.

[10] 董晓华,张丹参.大黄酚对Aβ(25-35)所致AD大鼠学习记忆及LTP的影响[J].中国药理学通报,2009,25(5):682-685.

[11] Benjamin B.Cho, Luis H.Toledo-Pereyra. Caspase-independent programmed cell death following ischemic stroke[J]. Invest Surg, 2008,21(3):141-147.

[12] Venkata Prasuja Nakka, Anchal Gusain, Suresh L.Mehta, et al. Molecular mechanisms of apoptosis in cerebral ischemia:multiple neuroprotective opportunities[J]. Mol Neurobiol,2008,37(1):7-38.

[13] M D’Amelio, V Cavallucci, F Cecconi. Neuronal caspase-3 signaling:not only cell death[J].Cell Death Differ,2010,17(7):1104–1114.

[14] Lucian Soane, Gary Fiskum. Inhibition of Mitochondrial Neural Cell Death Pathways by Protein Transduction of Bcl-2 Family Proteins[J]. Bioenerg Biomembr,2005,37(3):179–190.

[15] 周红艳,范军铭,王军.电针对局灶性脑缺血大鼠海马神经细胞凋亡及Bcl-2 Bax caspase-3表达的影响[J].辽宁中医杂志,2009,36(8):1427-1429.