ACTA NEUROPHARMACOLOGICA

ACTA NEUROPHARMACOLOGICA››2012,Vol. 2››Issue (5): 19-23.

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Construction of Cpn0147 Bait Vector in Yeast Two-hybrid from Chlamydia Pneumonia Potentially Causing Neurodegenerative Diseases

JIA Xiao-hui, TIAN Ying-xin, TIAN Min, HAN Xiao-dong, JIA Tian-jun

  1. Institute of Pathogen Biology and Immunology, Hebei North University, Zhangjiakou, 075000, China
  • Online:2012-10-26Published:2014-06-27
  • Contact:贾天军，男，教授，博士研究生，硕士生导师；研究方向：天然免疫与抗感染免疫；E-mail：jiantianjun@yahoo.com
  • About author:贾晓晖，男，实验师，硕士研究生；研究方向：抗感染免疫；E-mail：jiaxiaohuistudent@163.com

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Abstract:Objective:To construct a bait vector of pGBKT7-Cpn0147 for seeking its ligands with yeast two-hybrid system.Methods:Cpn0147 gene was amplified by polymerase chain reaction (PCR), and then cloned into pGBKT7 to construct the bait vector. These recombinant plasmids were transformed into yeast cells AH109 and Y187 to test toxic effects and self-activation activity.Results:The pGBKT7-Cpn0147 bait vector was constructed successfully, which neither had self-activation ability nor yeast cell toxicity.Conclusion: The constructed pGBKT7-Cpn0147 could be used as a bait vector in yeast two-hybrid system, and provided an experimental basis for further screening the interaction proteins.

Key words:chlamydia pneumoniae,inclusion,membrane protein,yeast ,,two-hybrid system,bait ,,vector,self-activation

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