Production of Microencapsulated Human Retinal Pigment Epithelial Cells by the High Voltage Electrostatic Method

Abstract

Abstract: Objective: To prepare alginate-chitosan-alginate（Alginate-Chitosan-Alginate, ACA） microencapsulated Human Retinal Pigment Epithelial（hRPE） cells using high voltage electrostatic method. Methods: The microencapsulated hRPE was prepared by using our home-made high voltage electrostatic system, and the total number and the survival rate of microencapsulated hRPE cells were studied in the culture media for 7 d. Results: hRPE cells ACA microcapsule with diameter of （400±13） μm were obtained. the total number and the survival rate of cells had no statistically significant differences at the first 3 d. But after 7 d the total number of cells and the survival number were increased and the survival rate of cells was reduced. Conclusion: hRPE cells ACA microcapsule can be obtained with high voltage electrostatic method; after 7 d, the microencapsulated cells grow faster.

Key words: high voltage electrostatic preparation method, microencapsulated, human reti-nal pigment epithelial Cell（hRPE）

CLC Number:

GAO Dong-xiao，ZHAO Ya-qun. Production of Microencapsulated Human Retinal Pigment Epithelial Cells by the High Voltage Electrostatic Method[J]. Acta Neuropharmacologica, 2011, 1(2): 24-27.

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