<strong>Protective Effect of Kaixin Powder Containing Serum on Corticosterone Induced CTX TNA2 Cells Injury</strong>

ACTA NEUROPHARMACOLOGICA››2014,Vol. 4››Issue (6): 1-5.

Protective Effect of Kaixin Powder Containing Serum on Corticosterone Induced CTX TNA2 Cells Injury

WEN Zhi-ling, WANG Zhen-zhen, HE Wen-bin, CHEN Nai-hong

Online:2014-12-26Published:2015-01-20
Contact:贺文彬，男，博士，研究生导师；E-mail:hewb@sxtcm.com 陈乃宏，男，，研究员，博士生导师；研究方向：分子神经药理学、中药药理学。E-mai:chennh@imm.ac.cn
About author:温智林，男，硕士研究生；研究方向:中医药防治抑郁症的基础研究。E-mai:627297979@qq.com
Supported by:

本研究由国家自然科学基金项目（No. 81274122、No. 81202507、No. 81273629），教育部博士点基金重点项目（No. 20121106130001），新药作用机制研究与药效评价北京市重点实验室资助项目（No. BZ0150），中央级公益性科研院所基本科研业务费，专项资金（No. 2014RC03），山西省回国留学人员科研资助项目（2013-134）、山西省国际科技合作项目（2010081065）资助。

Abstract

Abstract:Objective: To observe the effects of Kaixin powder containing serum on corticosterone induced injury of rat cortical astrocyte CTX TNA2 cell line.Methods: Firstly, SD rats were orally administered with Kaixin powder, positive drug fluoxetine (10 mg·kg-1) and solvent control in order to obtain corresponding serum. CTX TNA2 cell line injured by corticosterone was used to establish depression model in vitro and cell activity was detected with MTT. Then we tested whether Kaixin powder play a neuroprotective role through PI3K signaling pathway in this cell model with the inhibitors of this pathway and detected by western blotting analysis.Results: According to our data, when the corticosterone concentration reached 200 μM, the cell viability inhibition rate was stabilized at 60% with a well concentration dependent. Kaixin powder containing serum could significantly improve the survival rate of corticosterone injured CTX TNA2 cells. The western blotting results also showed that Kaixin powder containing serum could reverse the reduction of PI3K and AKT1/2 protein expression induced by corticosterone. However, after given the inhibitors of PI3K pathway, the protective effect of Kaixin powder was reversed.Conclusion: Kaixin powder containing serum has obvious protective effect on corticosterone injured CTX TNA2 cell line, and the mechanism may be associated with nerve cell protective related PI3K signal transduction pathway.

Key words:Kaixin powder,containing serum,corticosterone,PI3K,CTX TNA2

CLC Number:

R965

WEN Zhi-ling, WANG Zhen-zhen, HE Wen-bin, CHEN Nai-hong.Protective Effect of Kaixin Powder Containing Serum on Corticosterone Induced CTX TNA2 Cells Injury[J]. ACTA NEUROPHARMACOLOGICA, 2014, 4(6): 1-5.

References

[1]曾毅丹, 黄芳, 汪家梨. 开心散现代药理作用的研究[J]. 海峡药学, 2006, 18(5): 12-14.

[2]戴建国, 黄玉芳, 王中立, 等. 基于星形胶质细胞靶点的抑郁症发病机制研究进展[J]. 中国药理学通报, 2010, 26(9): 1132-1135.

[3]Sun Jian-Dong, Chen Nai-Hong, Yuan Yu-He, et al. Gap junction dysfunction in the prefrontal cortex induces depressive-like behaviors in rats[J]. Neuropsychopharmacology, 2012, 37(5): 1305–1320.

[4]李静, 陈红霞, 于能江, 等. 小补心汤总黄酮含药血清对皮质酮损伤神经细胞的保护作用[J]. 军事医学, 2011, 35(10): 765-768.

[5]温薇，黄树明, 刘明, 等. 开心散含药血清对A13诱发的PC 12细胞损伤的改善作用[J]. 中医药信息, 2012, 29(40): 80-81.

[6]Nikola Bridges, Karel Šlais, Eva Sykovál. The effects of chronic corticosterone on hippocampal astrocyte numbers: A comparison of male and female Wistar rats[J].Acta Neurobiol Experimentalis, 2008, 68(2): 131-138.

[7]Guo Wen-Zhi, Miao Yu-Liang, Zhang Hong, et al. Midazolam provides cytoprotective effect during corticosterone-induced damages in rat astrocytes by stimulating steroidogenesis [J].Neuroscience Letters, 2013, 547: 53-58.

[8]余冰颖. 开心散治疗CMS抑郁模型大鼠的作用及活性成分TenuifolisideA基于ERK和PI3KI3K通路介导的神经保护机制研究[D]. 张家口：欧宝平台登录 , 2013.

[9]Chen Lin, Dai Jian-guo, Wang Zhong-li, et al. Ginseng total saponins reverse corticosterone-induced changes in depression-like behavior and hippocampal plasticity-related proteins by interfering with GSK-3β-CREB signaling pathway. evidence-based complementary and alternative medicine[J].Neuroscience Letters, 2014, 2014: Article ID 506735, 11 pages.

[10]Silca R, Ana Raquel Mesquita, Joao M Bessa, et al. Lithium blocks stress-induced changes in depressive-like behavior and hippocampal cell fate: The role of glycogen-synthase-kinase-3β[J].Neuroscience, 2008, 152(3): 656–669.

[11] Danuta Jantas, S Krawczyk, W Lason. The predominant protective effect of tianeptine over other antidepressants in models of neuronal apoptosis: the effect blocked by inhibitors of MAPK/ERK1/2 and PI3-K/Akt pathways[J]. Neurotox Res, 2014, 25(2): 208-225.

[12]Jiang Bao-ping, Liu Ya-min, Le Liang, et al. Cajaninstilbene acid prevents corticosterone-induced apoptosis in PC12 cells by inhibiting the mitochondrial apoptotic pathway[J]. Cell Physiol Biochem, 2014, 34(3):1015-1026.

[13]Liu Ya-min, Shen Sheng-nan, Li Zong-yang, et al. Cajaninstilbene acid protects corticosterone-induced injury in PC12 cells by inhibiting oxidative and endoplasmic reticulum stress-mediated apoptosis[J].Neurochem Int, 2014, 78:43-52.

[14]赵海霞, 周小江, 刘屏, 等. 6种开心散类方对不同物质损伤神经细胞的保护作用[J]. 中国中药杂志, 2012, 11(37): 3472-3476.

[15] Zhu Ze-Hua, Fu Xin, Wu Gen-Cheng, et al. Astrocyte-conditioned medium protecting hippocampal neurons in primary cultures against corticosterone-induced damages via PI3-K/Akt signal pathway[J].Brain Research, 2006, 1114(1):1-10.

[16]Sreejata Chatterjee, Sujit K Sikdar. Corticosterone treatment results in enhanced release of peptidergic vesicles in astrocytes via cytoskeletal rearrangements[J].Glia, 2013, 61(12): 2050-2062.

Protective Effect of Kaixin Powder Containing Serum on Corticosterone Induced CTX TNA2 Cells Injury

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