Abstract:Objective: To study the protective effects of Radix Salviae Miltiorrhizae Hydrosoluble combinations on cerebral ischemia and reperfusion injury and its mechanism.Methods: Cerebral ischemia and reperfusion model was conducted referring to improved Himori method in which bilateral common carotid arteries were temporarily obstructed. The effect of combination on learning and memory were examined in Morris water maze test. Anoxia survival time of decapitated mice, the activities of SOD, CAT and ACheE, and the content of MDA were measured. The influences of combination were studied on the neurological function and the expression of apoptosis related protein caspase-3 in hippocampal neuron and nerve growth factor in cortex using immunohistochemistry.Results: Combinations (B2,C11) could obviously improve memory impairment induced by cerebral ischemia and reperfusion in mice, enhance hypoxia tolerance, increase the activities of SOD and CAT, with decreasing the activities of ACheE and the content of MDA. At the same time, the expression of capase-3 was inhibited, increasing NGF. Effect of combination were most obvious in group (B4 1.4μmol·L-1 ,C11 30μmol·L-1 ), A2 combination has no significant effect at any dose.Conclusion: Combinations (B4,C11) have a significantly protective effect on hippocampal neurons after cerebral ischemia and reperfusion injury. The mechanism may be related to removing free radicals, inhibiting apoptosis of nerve cells, and promoting nerve regeneration.

Key words:radix salviae miltiorrhizae,hydrosoluble constituents,ischemia reperfusion,learning and memory,cysteine-asparate protease-3,nerve growth factor