<strong>Application of Stereology in Neuroscience Research</strong>

Abstract

Abstract:Stereology is a science based on the principles of geometry and statistics. Recently, design-based stereology has been applied in the experimental research of neuroscience, which makes up the weakness of traditional histopathology in morphological quantitation. The questions of how to measure the volume of regions of interest (ROI), the number of cells (neurons and glial cells) within ROI, the average volume of these cells and their subcellular structure (nuclear, mitochondria), the length of linear biological structures (nerve ?bers and vessels), and the spatial distribution of positive cells within ROI have gradually become the research focus. This article reviewed the application of design-based stereology in neuroscience research, the requirements to the experimental equipments, the sampling techniques and the directions of sectioning in stereological approach, and the commonly used stereological parameters.

Key words:design-based stereology,quantitation measurement,region of interest,central nervous system

CLC Number:

R964

TANG Wen-jing, MA Deng-lei, LI lin.Application of Stereology in Neuroscience Research[J]. Acta Neuropharmacologica, 2014, 4(5): 24-29.

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Application of Stereology in Neuroscience Research

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