<strong>Effect of Kangnaoye on Expression of VEGF, BDNF, MMP-9 after Cerebral Ischemia Reperfusion in Rats</strong>

ACTA NEUROPHARMACOLOGICA››2014,Vol. 4››Issue (2): 16-21.

Effect of Kangnaoye on Expression of VEGF, BDNF, MMP-9 after Cerebral Ischemia Reperfusion in Rats

MA Jian-peng, CHANG-Qing, XUE-Qian, ZOU Yu-an

1 Hebei North University, Zhangjiakou, 075000, China
2 Department of Neurology, the First Affiliated Hospital of Hebei North University, Zhangjiakou, 075000, China

Online:2014-04-26Published:2014-06-27
Contact:薛茜,女,教授,主任医师,硕士,硕士生导师;研究方向:缺血脑血管病;E-mail:xueqian6166@163.com
About author:马建鹏,女,硕士研究生在读；研究方向：缺血性脑血管病；E-mail：majianpeng45@163.com
Supported by:

河北省卫生厅课题基金资助项目( 20090591)

Abstract

Abstract:Objective: To observe the effect of Kangnaoye pretreatment on the expression of vascular endothelial growth factor (VEGF), brain derived neurotrophic factor (BDNF),matrix metalloproteinase-9 (MMP-9) in rats with cerebral ischemia-reperfusion injury, and further to explore the neurovacular unit protective mechanism of Kangnaoye against the injury of cerebral ischemia-reperfusion.Methods: Male Spraque-Dawley rats were randomly divided into: Sham operation group，ischemia-reperfusion group，Kangnaoye 28.6，14.3，7.15 g·kg -1·d-1 dose group (intragastric administration, 7 d). Improved Zea Longa method was used to prepare cerebral ischemia-reperfusion model of rats. Reperfusion after ischemia for 2 h（rats were sacrificed respectively after reperfusion 6 h，12 h，24 h，72 h，7 d）. TTC staining method was used for observing the infarct area of rat brain; Immunohistochemical method was used for observing the expression of VEGF, BDNF, MMP-9 in rats brain tissue.Results: The comparison of the infarct area between the groups 24h after reperfusion ischemic found that the Kny High, Middle dose group significantly decreased the infarct area (P<0.05), along with the unregulated expression of VEGF,BDNF（P<0.01）. The expression of MMP-9 was significantly decreased as compared to model group（P<0.01）.Conclusion: Kangnaoye promoted the expression of VEGF, BDNF in brain tissue, inhibited the expression of MMP-9, decreased the infarct area and protected neurovascular unit against cerebral ischemia reperfusion injury.

Key words:kangnaoye,neurovascular unit,vascular endothelial growth factor,brain derived neurotrophic factor,matrix metalloproteinase-9

CLC Number:

R965

MA Jian-peng, CHANG-Qing, XUE-Qian, ZOU Yu-an.Effect of Kangnaoye on Expression of VEGF, BDNF, MMP-9 after Cerebral Ischemia Reperfusion in Rats[J]. ACTA NEUROPHARMACOLOGICA, 2014, 4(2): 16-21.

References

[1] Sun Xiu-lan, Hu Gang. ATP-sensitive potassium channels: a promising target for protecting neurovascular unit function in stroke[J].Clin Exp Pharmacol physiol, 2010, 37(2): 243-252.

[2] Gregory J del Zoppo. Stroke and neurovascular protection[J].N Engl J Med, 2006, 354(6): 553-555.

[3] 周赛男,蔺晓源,易健,等.补阳还五汤对脑缺血大鼠神经功能及细胞形态的影响[J].中国实验方剂学杂志, 2013, 19(2): 251-254.

[4] 石会娟,梁起保,薛茜,等.康脑液对脑缺血再灌注大鼠血清及脑组织中氨基酸含量的影响[J].天津医药, 2011, 39(10): 939-941.

[5] 赵志敏,肖志娟,薛茜,等.康脑液对脑缺血再灌注动物血管新生的影响[J].中国临床药理学, 2014, 30(10): 429-431.

[6] Wang Yong-quan, Guo Xin, Qiu Mei-hong, et al. VEGF overexpression enhances striatal neurogenesis in brain of adult rat after a transient middle cerebral artery occlusion[J].J Neurosci Res, 2007, 85(1): 73-82.

[7] Jin Kun-lin, Zhu Yong-hua, Sun Yun-juan, et al. Vascular endothelial growth factor (VEGF) stimulates neurogenesis in vitro and in vivo[J].Proc Natl Acad Sci USA, 2002, 99(18): 11946-11950.

[8] Feng S, Cen J, Huang Y, et al. Matrix metalloproteinase-2 and -9 secreted by leukemic cells increase the permeability of blood-brain barrier by disrupting tight junction proteins [J]. PLoS One, 2011, 6(8): e20599.

[9] Enrique Zea Longa, Philip R Weinstein, Sara Carlson, et al. Reversible middle cerebral artery occlusion without cranietomy in rats[J].Stroke, 1989, 20(1):84-91.

[10] Joshua B Bederson, Lawrence H Pitts, Miles Tsuji, et al. Rat middle cerebral artery occlusion: evaluation of the model and development of a neurologic examination [J].Stroke, 1986, 17(3): 472-476．

[11] Otilia M?rg?ritescu, Pirici D, M?rg?ritescu C L.VEGF expression in human brain tissue after acute ischemic stroke[J].Rom J Morphol Embryol, 2011, 52(4): 1283-1292.

[12] 赛音巴雅尔,林贵军,饶芝国,等.外源性血管内皮生长因子对大鼠脑血肿周围血管新生及神经功能恢复的影响[J].中华神经外科杂志, 2012, 28(5): 522-525.

[13] Guo Shu-zhen, Woo Jean Kim, Josephine Lok, et al. Neuropretection via matrix-troohic coupling between cerebral cells and neurons[J].Proc Natl Acad Sci USA, 2008, 105(21): 7582-7587.

[14] Hyun Kim, Li Qi, Barbara L Hempstead, et al. Paracrine and autocrine functions of brain- derived neurotrophic factor (BDNF)and nerve growth factor (NGF) in brain-der ivedendothe lial cells [J]. J Bio l Chem, 2004, 279(32): 33538-33546.

[15] 王强,张一,王榕,等.丹参多酚酸盐对缺血再灌注损伤大鼠脑组织BDNF和GDNF的影响[J].卒中与神经疾病, 2013, 5(20): 272-274.

[16] Eng H Lo, Turgay Dalkara, Michael A Moskowitz. Mechanisms, challenges and opportunities in stroke [J]. Nat Rev Neurosci, 2003, 4(5): 399-415.

[17] Li Hong, Wei Ming, Li Sheng-hui, et al. Increased CD147 and MMP-9 expression in the normal rat brain after gamma irradiation[J]. J Radiat Res, 2013, 54(1):27-35.

Effect of Kangnaoye on Expression of VEGF, BDNF, MMP-9 after Cerebral Ischemia Reperfusion in Rats

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles3

Recommended Articles

Metrics

[1]	ZHANG Qun-feng，HE Huan-ying，LIAO Yun-hao，WANG Guo-qing.The Effects of Maternal Chlorpyrifos Exposure on the Expression of COX2，CREB and BDNF in Hippocampus of Offspring Mice[J]. Acta Neuropharmacologica, 2019, 9(6): 1-5.
[2]	SUN Yan，SONG Ai-xia，XUE Qian，ZOU Yu-an.Effect of Kangnaoye on Nerve Regeneration Related Factor in Rats of Cerebral Ischemia-Reperfusion Injury[J]. Acta Neuropharmacologica, 2015, 5(5): 7-14.
[3]	WANG Hao, DU Guan-tao, LIU Guang-jun, HONG Hao.Research Progress on RAGE/LRP1 Transporters At the Blood-Brain Barrier and the Neurovascular Unit in Alzheimer’s Disease[J]. Acta Neuropharmacologica, 2015, 5(2): 38-45.