Abstract:Objective: To study purification and anti-oxidant activity of the polysaccharide Isolated from Trolliouschinensis. This study provides basical information to develop and utilize Trollious chinensis resource efficiently.Methods: 1 The methods of reflux extraction was adapted to extract polysaccharide from Trollius chinensis, AB-8 resin can be used as a high effective one to romove pigment and protein of Trollius chinensis polysaccharide(TCBP). A DEAE-52 cellulose ion exchange column ，a Sephadex G-150 gels filter column were successively used to further separate crude polysaccharides, and then determining the product purity and molecular weight with high performance gels filtration chromagraphy(HPGPC). High performance liquid Chromatography(HPLC) was applied to study the momosaccharide composition of Trollius chinensis polysaccharide. 2 Antioxidative capability of polysaccharide was determined by salicylic-Fe2+-H2O2 system(·OH), 1,1-Diphenyl-2-picrylhydrazyl radical 2, 2-Diphenyl-1-(2, 4, 6-trinitrophenyl)hydrazyl(DPPH) system, and means of pyrogallol oxidation（·O2－）.Results: 1 Our results showed that four polysaccharides are complex polysaccharide with a variety of monosaccharides. Molecular weight of TCBP1 is 3283220 Da, TCBP2, 156819 Da, TCBP3, 10949 Da and TCBP4, 1068 Da. 2 Four polysaccharides had significant effects on scavenging hydroxyl radical, The sequence from strong to weak of this effects is: TCBP2 > TCBP3 > TCBP1 > TCBP4. TCBP2 and TCBP3 had certain effect on 1, 1-Diphenyl -2- picrylhydrazyl radical. They all induced superoxide radical instead of scavenging.Conclusion: TCBP1, TCBP2, TCBP3 and TCBP4 are complex polysaccharide with a variety of monosaccharides，they have the certain antioxidant activity.

Key words:trollius chinensis,polysaccharide,isolation and purification,anti-oxidation