<strong>Protective Effect of Astragaloside Ⅳ on PC12 Cells Injured by Oxygen-glucose Deprivation</strong>

Abstract

Abstract:Objective：To investigate the protective effect and mechanism of astragaloside Ⅳ on PC12 cells injured by oxygen-glucose deprivation.Methods：The oxygen-glucose deprivation model of PC12 cells was constructed with Na2S2O4 diluted with glucose-free Earle’s. PC12 cells were treated with different doses of astragaloside Ⅳ. Nimodipine was taken as a positive control. The cellular morphology difference was observed and the cell survival rate was detected with MTT method. The lactate dehydrogenase（ LDH） concentration was determined with enzyme linked immunosorbent assay. The expression of Bcl-2 and Bax protein were detected by Western blot.Results：The oxygen glucose deprivation（ OGD） model was successfully established. In contrast with the OGD group，astragaloside Ⅳ could increase the survival rate of PC12 cells and reduce LDH leakage. Astragaloside Ⅳ could also increase the expression of Bcl-2 protein and decrease the expression of Bax protein.Conclusion：Astragaloside Ⅳ could relieve the PC12 cell injury induced by OGD and the protective effect may be related to inhibiting cell apoptosis.

Key words:Astragaloside Ⅳ,PC12 cell,Oxygen glucose deprivation,Apoptosis

CLC Number:

R965

YANG Wen-liang, SONG Yu, ZHAO Can-can, XIE Xin-mei, PANG Xiao-bin, DU Guan-hua.Protective Effect of Astragaloside Ⅳ on PC12 Cells Injured by Oxygen-glucose Deprivation[J]. ACTA NEUROPHARMACOLOGICA, 2015, 5(1): 10-14.

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Protective Effect of Astragaloside Ⅳ on PC12 Cells Injured by Oxygen-glucose Deprivation

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