<strong>Inf luence of Saff lower Injection on Expression of Bax and Bcl-XL/Bcl-2 Protein in the Cortex of Rat after Ischemia-Reperfusion Injury</strong>

ACTA NEUROPHARMACOLOGICA››2015,Vol. 5››Issue (1): 15-18.

Inf luence of Saff lower Injection on Expression of Bax and Bcl-XL/Bcl-2 Protein in the Cortex of Rat after Ischemia-Reperfusion Injury

CHEN Qian, FENG Fei, CHEN Wen-ming, YANG Xiao-yan, LUO Yong

1. Department of Neurology, The Third Affiliated Hospital of Zunyi Medical College, The First People’s Hospital of ZunYi, ZunYi, 563002, China
2. Laboratory of Internal Medicine, The First Affiliated Hospital of Zunyi Medical College, ZunYi, 563003, China
3. Department of Neurology, Guizhou Aerospace Hospital, ZunYi, 563003, China

Online:2015-02-26Published:2015-07-06
Contact:罗勇，男，主任医师，硕士研究生导师；研究方向：神经药理及脑血管疾病，E-mail: luoyongtt@163.com
About author:陈乾，男，硕士研究生；E-mail：2207340469@qq.com
Supported by:

贵州省科学技术基金项目（黔科合J字[2009]2155号），贵州省卫生厅科学技术基金项目（gzwkj2009-1-052）

Abstract

Abstract:Objective：To explore the influence of safflower injection on expression of Bax， Bcl-2 and Bcl-XL protein in the cortex of rat after ischemia-reperfusion injury.Methods：30 healthy male SD rats were randomly divided into six groups，as indicated，control group，model control group，safflower injection low dose group（ 0.8 mL·kg-1），middle dose group（ 1.6 mL·kg-1），high dose group（ 3.2 mL·kg-1） and Nimodipine group（ 2.0 mL·kg-1）. The focal cerebral ischemia-reperfusion injury model was established by thread technique，and the expression of Bax，Bcl-2 and Bcl-XL protein in the rat brain cortex was detected through immunohistochemical methods.Results：We found that the expression of positive cells of Bax，Bcl-2 and Bcl-XL protein was signifi cantly increased in the surrounding area of the ischemic brain tissue in the model group，compared with the control group（ P<0.05）； and the expression of positive cells of Bax protein was reduced and the expression of Bcl-2，Bcl-XL protein was rised in the surrounding area of the ischemic brain tissue in the safflower injection low dose group，middle dose group，high dose group and Nimodipine group，compared with the model group（ P<0.05）.Conclusion：Safflower injection can protect cerebral neurons from apoptosis through augmenting the expresson of Bcl-2，Bcl-XL and decreasing the expression of Bax protein after ischemia-reperfusion injury.

Key words:safflower injection,ischemia-reperfusion injury,Bcl-2,Bcl-XL,Bax

CLC Number:

R965

CHEN Qian, FENG Fei, CHEN Wen-ming, YANG Xiao-yan, LUO Yong.Inf luence of Saff lower Injection on Expression of Bax and Bcl-XL/Bcl-2 Protein in the Cortex of Rat after Ischemia-Reperfusion Injury[J]. ACTA NEUROPHARMACOLOGICA, 2015, 5(1): 15-18.

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Inf luence of Saff lower Injection on Expression of Bax and Bcl-XL/Bcl-2 Protein in the Cortex of Rat after Ischemia-Reperfusion Injury

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