Acta Neuropharmacologica››2015,Vol. 5››Issue (4): 22-25.

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The Skills of Intracerebral Hemorrhage Rat Model by Type VII Collagenase Injection

LI Dong-fang, ZHAO Hai-bin, JIANG Tian-yuan, ZHAI Yao-yao, YANG Dan-dan

  1. The Third Affiliated Hospital of Beijing University of Traditional Chinese Medicine, Beijing, 10029, China
  • Online:2015-08-26Published:2016-03-03
  • Contact:赵海滨,男,医学博士后,教授,博士生导师;研究方向:中医药防治心脑血管疾病; Tel:+86-010-52075251,E-mail:haibin999@126.com
  • Supported by:

    国家自然基金项目(No.81373590)

Abstract:Objective: To study the skills of intracerebral hemorrhage model by injecting type Ⅶ collagenase into brain region in rats.Methods: The animal model of intracerebral hemorrhage was induced by the injection of type Ⅶ collagenase into caudate nucleus of rats with stereotactic instrument and improved methods. And then evaluate the feasibility and stability of the model through behavioral assessment initially.Results: we had made 150 rat models, two rats died, nine rats was not successful. The success rate of intracerebral hemorrhage model was 92.7%.Conclusion: The method of injecting type Ⅶ collagenase can be used to build the animal model of intracerebral hemorrhage ideally and steadily. Because of the simple experiment operation, it has a great promotional vaule.

Key words:collagenase,rat,intracerebral hemorrhage,model

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