Abstract:Objective：To explore the dynamic anti-oxidative effects of chrysophanol lipidosome on cerebral ischemia-reperfusion injury in mice.Methods：Mice were subjected to middle cerebral artery occlusion by suture method，then they were randomly divided into the control group，sham group，model group，nimodipine group （1.0 mg·kg-1） and chrysophanol lipidosome treatment （10.0，5.0，0.5 mg·kg-1） groups. The control group，sham group，modelgroup were injected with normal saline （10 mL·kg-1，ip）. Chrysophanol lipidosome treatment groups intraperitoneally were injected with chrysophanol lipidosome （10.0，5.0，0.5 mg·kg-1） before cerebral ischemia one hour，once a day，then the mice were divided into 6 subgroups according to different reperfusion time points，3，6，12，24，48，72 hour. According to the different time points，neurological deficits，brain water content，infarct volume were determined；the activity of NOS，SOD，GSH-PX，the content of MDA，NO were determined by the kits methods.Results：Compared with sham group，in model group the neurological deficits，brain water content，infarct volume were increased （P<0.01）；the activity of NOS and the content of MDA，NO were increased；the activity of SOD and GSH-PX were reduced （P<0.01）. Chrysophanol lipidosome reduced the neurological deficits，brain water content，infarct volume，the content of MDA，NO，and activity of NOS，and elevated the activities of SOD，GSH-PX （P<0.05）.Conclusion：So the protective effect of chrysophanol lipidosome on cerebral ischemiareperfusion injury might be involved in its anti-oxidative.

Key words:chrysophanol lipidosome,cerebral ischemia-reperfusion,anti-oxidative