ACTA NEUROPHARMACOLOGICA››2011,Vol. 1››Issue (5): 1-7.

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Protective Effect of PPT and Ginsenoside-Re in MPP+-treated SH-SY5Y Cells

LI Dong-wei,DOU De-qiang

  1. College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, 116620, China
  • Online:2011-10-26Published:2013-03-24
  • Contact:窦德强,男,教授,博士研究生导师;Tel:+86-0411-87406497,E-mail:doudeqiang7003@yahoo.com.cn
  • About author:李东薇,女,硕士研究生;研究方向:中药药效物质基础;E-mail:wei-5599@163.com

Abstract:Objective:To investigate the protective effect of PPT (protopanaxatriol group) and ginsenoside-Re on 1-methyl-4-phenylpyridinium ion (MPP+)-induced damage in SH-SY5Y(human dopaminergic neuroblastoma cell line)cells.Methods:SH-SY5Y cells were treated with different concentrations of PPT, ginsenoside-Re or MPP+ containing serum. MTT colorimetry was used to test the viability of the cells and the survival rates were calculated and analyzed.Results:Cells treated by either 5 µmol•L-1, 10 µmol•L-1 PPT or ginsenoside-Reshowed significantly higher viability as compared to control group (P<0.05). In addition, 10% PPT treated or ginsenoside-Remedicated serum treated groups showed similar protective effect.Conclusion:PPT and ginsenoside-Re have neuroprotective effects in a cell model of Parkinson’s disease MPP+-induced SH-SY5Y cell death, suggesting that both PPT and ginsenoside-Re may have anti-PD effect.

Key words:protopanaxatriol group,ginsenoside-Re,SH-SY5Y cells,Parkinson&,rsquo,s disease,,neuroprotective effects

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