<strong>Total Saponins from <em>Rhizoma Anemarrhenae</em> Increase Acetylcholinesterase Activity in the Hippocampus of Diabetic Rats</strong>

Abstract

Abstract:Objective:To investigate the effects of total saponins fromRhizoma Anemarrhenae(TS) on acetylcholinesterase (AchE) activity in the hippocampus of streptozotocin-induced diabetic rats and their possible mechanisms.Methods:Diabetic rats were treated with TS (200 mg?kg^-1, ig.) for 8 weeks, and their blood glucose and body weight were measured monthly and weekly, respectively. The hippocampus was obtained for assays of AchE activity as well as levels of malondialdehyde (MDA) and reduced glutathione (GSH); blood was collected for assays of levels of MDA, GSH and glucose.Results:Compared with that of the age-matched normal rats, AchE activity was markedly increased in the hippocampus of diabetic rats, and TS treatment blocked such an increase. Meanwhile, diabetes significantly elevated the MDA level and reduced GSH level both in the hippocampus and the serum, and TS treatment significantly prevented such changes. In addition, TS significantly decreased the fasting blood glucose level and increased the body weights of diabetic rats.Conclusion:TShas an inhibitory effect on AchE activity in the hippocampus in diabetic rats, which is at least partly due to its antioxidant and anti-diabetic activity.

Key words:diabetes mellitus,acetylcholinesterase,Rhizoma Anemarrhenae,saponins,oxidative stress,blood glucose-lowering

CLC Number:

R965

ZHAI Yun-peng, ZHU Xia, LU Qian, WANG Jian-yun, YIN Xiao-xing, LIU Yao-wu.Total Saponins fromRhizoma AnemarrhenaeIncrease Acetylcholinesterase Activity in the Hippocampus of Diabetic Rats[J]. Acta Neuropharmacologica, 2012, 2(1): 1-9.

References

[1] Biessels GJ, Deary IJ, Ryan CM. Cognition and diabetes: a lifespan perspective[J]. Lancet Neurol, 2008, 7(2):184-190.

[2] Christopher T Kodl, Elizabeth R Seaquist. Cognitive dysfunction and diabetes mellitus[J]. Endocr Rev, 2008, 29(4):494-511.

[3] Shayna A Wrighten, Gerardo G Piroli, Claudia A Grillo, et al. A look inside the diabetic brain: contributors to diabetes-induced brain aging[J]. Biochim Biophys Acta, 2009, 1792(5):444-453.

[4] Mastrocola R, Restivo F, Vercellinatto I, et al. Oxidative and nitrosative stress in brain mitochondria of diabetic rats[J]. J Endocrinol, 2005, 187(1):37-44.

[5] Alette M Wessels, Philip Scheltens, Frederk Barkhof, et al. Hyperglycaemia as a determinant of cognitive decline in patients with type 1 diabetes[J]. Eur J Pharmacol, 2008, 585(1):88-96.

[6] Michael Brownlee. Biochemistry and molecular cell biology of diabetic complications[J]. Nature, 2001, 414(6865):813-820.

[7] Nigel A Calcutt, Mark E Cooper, Tim S Kern, et al. Therapies for hyperglycaemia-induced diabetic complications: from animal models to clinical trials[J]. Nat Rev Drug Discov, 2009, 8(5):417-429.

[8] Ryan CM, Geckle MO, Orchard TJ. Cognitive efficiency declines over time in adults with type 1 diabetes: effects of micro- and macrovascular complications[J]. Diabetologia, 2003, 46(7):940-948.

[9] Jacobson AM, Ryan CM, Cleary PA, et al. Biomedical risk factors for decreased cognitive functioning in type 1 diabetes: an 18 year follow-up of the diabetes control and complications trial (DCCT) cohort[J]. Diabetologia, 2011, 54(2):245-255.

[10] Anurag Kuhad, Kanwaljit Chopra. Curcumin attenuates diabetic encephalopathy in rats: behavioral and biochemical evidences[J]. Eur J Pharmacol, 2007, 576(1-3):34-42.

[11] Gunjan Saxena, Sheelendra Pratap Singh, Raghvendra Pal, et al. Gugulipid, an extract of commiphora whighitii with lipid-lowering properties, has protective effects against streptozotocin-induced memory deficits in mice[J]. Pharmacol Biochem Behav, 2007, 86(4):797-805.

[12] Pravinkumar Bhutada, Yogita Mundhada, Kuldeep Bansod, et al. Protection of cholinergic and antioxidant system contributes to the effect of berberine ameliorating memory dysfunction in rat model of streptozotocin-induced diabetes[J]. Behav Brain Res, 2011, 220(1):30-41.

[13] Anurag Kuhad, Mahendra Bishnoi, Vinod Tiwari, et al. Suppression of NF-kappabeta signaling pathway by tocotrienol can prevent diabetes associated cognitive deficits[J]. Pharmacol Biochem Behav, 2009, 92(2):251-259.

[14] Roberta Schmatz, Cinthia Melazzo Mazzanti, Roselia Spanevello, et al. Resveratrol prevents memory deficits and the increase in acetylcholinesterase activity in streptozotocin-induced diabetic rats[J]. Eur J Pharmacol, 2009, 610(1-3):42-48.

[15] Abdel Khalek Mohamed, Angelika Bierhaus, Stephan Schiekofer, et al. The role of oxidative stress and NF-kappaB activation in late diabetic complications[J].Biofactors, 1999, 10(2-3):157-167.

[16] Berr C, Balansard B, Arnaud J, et al. Cognitive decline is associated with systemic oxidative stress: the EVA study. etude du vieillissement arteriel[J]. J Am Geriatr Soc, 2000, 48(10):1285-1291.

[17] Koji Fukui, Koji Onodera, Tadashi Shinkai, et al. Impairment of learning and memory in rats caused by oxidative stress and aging, and changes in antioxidative defense systems[J]. Ann N Y Acad Sci, 2001, 928:168-175.

[18] Paul G Layer, Regina Alber, Olaf Sporns. Quantitative development and molecular forms of acetyl- and butyrylcholinesterase during morphogenesis and synaptogenesis of chick brain and retina[J]. J Neurochem, 1987, 49(1):175-182.

[19] Ariel E Reyes, Daniel R Perez, Alejandra Alvarez, et al. A monoclonal antibody against acetylcholinesterase inhibits the formation of amyloid fibrils induced by the enzyme[J]. Biochem Biophys Res Commun, 1997, 232(3):652-655.

[20] Anurag Kuhad, Kanwaljit Chopra. Effect of sesamol on diabetes-associated cognitive decline in rats[J]. Exp Brain Res, 2008, 185(3):411-420.

[21] Anurag Kuhad, Richa Sethi, Kanwaljit Chopra. Lycopene attenuates diabetes-associated cognitive decline in rats[J]. Life Sci, 2008, 83(3-4):128-134.

[22] Yasuhiro Iida, Ki-Bong Oh, Mikako Saito, et al. Detection of antifungal activity in anemarrhena asphodeloides by sensitive BCT method and isolation of its active compound[J]. J Agric Food Chem, 1999, 47(2):584-587.

[23] Zhang Jian-ying, Meng Zhi-yun, Zhang Mei-ying, et al. Effect of six steroidal saponins isolated from anemarrhenae rhizoma on platelet aggregation and hemolysis in human blood[J]. Clin Chim Acta, 1999, 289(1-2):79-88

[24] Zhang Jianying, Zhang Meiying, Kazunori Sugahara, et al. Effect of steroidal saponins of anemarrhenae rhizoma on superoxide generation in human neutrophils[J]. Biochem Biophys Res Commun, 1999, 259(3):636-639.

[25] Hyun-Shiek Yeum, Young-Cheol Lee, Seung-Hyun Kim, et al. Fritillaria cirrhosa, anemarrhena asphodeloides, lee-mo-tang and cyclosporine a inhibit ovalbumin-induced eosinophil accumulation and Th2-mediated bronchial hyperresponsiveness in a murine model of asthma[J]. Basic Clin Pharmacol Toxicol, 2007, 100(3):205-213.

[26] Bomi Lee, Hien Trung Trinh, Kangsik Jung, et al. Inhibitory effects of steroidal timosaponins isolated from the rhizomes of anemarrhena asphodeloides against passive cutaneous anaphylaxis reaction and pruritus[J]. Immunopharmacol Immunotoxicol, 2010, 32(3):357-363.

[27] Xie Wei-dong, Zhao Yun-an, Zhang Yaou. Traditional Chinese medicines in treatment of patients with type 2 diabetes mellitus[J]. Evid Based Complement Alternat Med, 2011, 2011:726723.

[28] 陈勤, 夏宗勤, 胡雅儿. 知母皂苷元对拟痴呆大鼠β-淀粉样肽沉积及胆碱能系统功能的影响[J].中国药理学通报, 2002,18(4):390-393.

[29] Yaer Hu, Xia Zong-qin, Sun Qi-xiang, et al. A new approach to the pharmacological regulation of memory: sarsasapogenin improves memory by elevating the low muscarinic acetylcholine receptor density in brains of memory-deficit rat models[J]. Brain Res, 2005, 1060(1-2):26-39.

[30] Ouyang Shi, Sun Li-sha, Guo Sheng-lan, et al. Effects of timosaponins on learning and memory abilities of rats with dementia induced by lateral cerebral ventricular injection of amyloid beta- peptide[J]. Di Yi Jun Yi Da Xue Xue Bao, 2005, 25(2):121-126.

[31] Li Tie-jun, Qiu Yan, Yang Peng-yuan, et al. Timosaponin B-II improves memory and learning dysfunction induced by cerebral ischemia in rats[J]. Neurosci Lett, 2007, 421(2):147-151.

[32] Bomi Lee, Kangsik Jung, Dong-Hyun Kim. Timosaponin AIII, a saponin isolated from anemarrhena asphodeloides, ameliorates learning and memory deficits in mice[J]. Pharmacol Biochem Behav, 2009, 93(2):121-127.

[33] 欧阳石, 孙莉莎, 徐江平. 知母总皂苷对大鼠脑皮质乙酰胆碱酯酶的抑制作用[J]. 中国药学杂志, 2006, 41(19):1472-1474.

[34] Liu Yao-wu, Zhu Xia, Lu Qian, et al. Total saponins from rhizoma anemarrhenae ameliorate diabetes-associated cognitive decline in rats: involvement of amyloid-beta decrease in brain[J]. J Ethnopharmacol, 2012, 139(1):194-200.

[35] George L Ellman, K.Diane Courtney, Valentino Andres Jr, et al. A new and rapid colorimetric determination of acetylcholinesterase activity[J]. Biochem Pharmacol, 1961, 7(2):88-95.

[36] Wills ED. Mechanisms of lipid peroxide formation in animal tissues[J]. Biochem J, 1966, 99(3):667-676.

[37] Jollow DJ, Mitchell JR, Zampaglione N, et al. Bromobenzene-induced liver necrosis. protective role of glutathione and evidence for 3,4-bromobenzene oxide as the hepatotoxic metabolite[J]. Pharmacology, 1974, 11(3):151-169.

[38] Anders A Sima. Encephalopathies: the emerging diabetic complications[J]. Acta Diabetol, 2010, 47(4):279-293.

[39] Gian Sberna, Javier Saez-Valero, Li Qiao-xin, et al. Acetylcholinesterase is increased in the brains of transgenic mice expressing the C-terminal fragment (CT100) of the beta-amyloid protein precursor of Alzheimer's disease[J]. J Neurochem, 1998, 71(2):723-731.

[40] Rakonczay Z, Vincendon G, Zanetta JP. Heterogeneity of rat brain acetylcholinesterase: a study by gel filtration and gradient centrifugation[J]. J Neurochem, 1981, 37(3):662-669.

[41] Doaa A Ghareeb, Hend M Hussen. Vanadium improves brain acetylcholinesterase activity on early stage alloxan-diabetic rats[J]. Neurosci Lett, 2008, 436(1):44-47.

[42] Cambay Z, Baydas G, Tuzcu M, et al. Pomegranate (Punica granatum L.) flower improves learning and memory performances impaired by diabetes mellitus in rats[J]. Acta Physiol Hung, 2011, 98(4):409-420.

[43] Sandhir R, Julka D, Gill KD. Lipoperoxidative damage on lead exposure in rat brain and its implications on membrane bound enzymes[J]. Pharmacol Toxicol, 1994, 74(2):66-71.

Total Saponins fromRhizoma AnemarrhenaeIncrease Acetylcholinesterase Activity in the Hippocampus of Diabetic Rats

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