<strong>Transient Receptor Potential Melastatin 7 Involved in the Proliferation Induced by Platelet Derived Growth Factor and 5-HT in Pulmonary Artery Smooth Muscle Cells of Rates</strong>

Acta Neuropharmacologica››2012,Vol. 2››Issue (3): 15-21.

Transient Receptor Potential Melastatin 7 Involved in the Proliferation Induced by Platelet Derived Growth Factor and 5-HT in Pulmonary Artery Smooth Muscle Cells of Rates

GAO Hai-xia¹, QIAO Xiao-wen², DUN Jie-ning³, DONG Ming-gang⁴，XU Zhi-wei⁴, ZHANG Hai-lin¹

1 The Key Laboratory of Neural and Vascular Biology, Ministry of Education, Department of Pharmacology, Hebei Medical University, Shijiazhuang, 050000, China
2 Department of Pediatrics, Xinji Hospital of Traditional Chinese Medicin, 052360, China

3 Department of Pharmacy, Hebei Medical University, Shijiazhuang, 050000, China
4 Hebei North University, Zhangjiakou, 075000, China

Online:2012-06-26Published:2013-12-25
Contact:张海林，河北医科大学药理学教研室；E-mail：z.hailin@yahoo.com
About author:杲海霞，河北医科大学药理学教研室；Tel+86-0311-86261032，E-mail：gaohx686@yahoo.com
Supported by:

国家自然科学基金青年基金（No.81201642）

Abstract

Abstract:Objective: To investigate the role of transient receptor potential melastatin7 (TRPM7) in platelet derived growth factor (PDGF) and 5-hydroxytryptamine (5-HT)-induced rat pulmonary artery smooth muscle cells (PASMCs) abnormal proliferation.Methods:PASMCAs were cultured with the explant technique. The expression of TRPM7 was evaluated by RT-PCR, western blot and whole-cell patch clamp. The proliferation of PASMCs was evaluated by MTT assay. TRPM7 inhibitor 2-APB was used to investigate the receptor mechanism of TRPM7-like current and the effects of TRPM7 in abnormal proliferation induced by PDGF and 5-HT.Results: RT-PCR and western blot showed that the TRPM7 mRNA and protein were detected in rat PASMCs. Electrophysiological results showed that the TRPM7 currents were detectable and that PDGF can increase the amplitude of TRPM7 inside and outside currents. 100 μmol•L^-12-APB inhibited PDGF and 5-HT-induced abnormal proliferation in rat PASMCs.Conclusion: TRPM7 participates in PDGF and 5-HT-induced abnormal proliferation in rat PASMCs and TRPM7 may be a new target for clinical treatment of pulmonary artery hypertension (PHA).

Key words:transient receptor potential melastatin7 (TRPM7),platelet derived growth factor (PDGF),5-hydroxytryptamine (5-HT),platelet derived growth factor (PASMCs),pulmonary artery hypertension (PAH)

CLC Number:

R349.5

GAO Hai-xia, QIAO Xiao-wen, DUN Jie-ning, DONG Ming-gang，XU Zhi-wei,ZHANG Hai-lin.Transient Receptor Potential Melastatin 7 Involved in the Proliferation Induced by Platelet Derived Growth Factor and 5-HT in Pulmonary Artery Smooth Muscle Cells of Rates[J]. Acta Neuropharmacologica, 2012, 2(3): 15-21.

References

[1] Yogi A, Callera GE, O'Connor S, et al. Aldosterone signaling through transient receptor potential melastatin 7 cation channel (TRPM7) and its alpha-kinase domain [J]. Cell Signal, 2013, doi: 10.1016/j.cellsig.2013.07.002.

[2] Yu Ying, Sweeney Michele, Zhang Shen, et al. PDGF stimulates pulmonary vascular smooth muscle cell proliferation by upregulating TRPC6 expression [J]. Am J Physiol Cell Physiol, 2003, 284(2): C316-C330.

[3] Sun Hong-Shuo, Michael F Jackson, Loren J Martin, et al. Suppression of hippocampal TRPM7 protein prevents delayed neuronal death in brain ischemia [J]. Nat Neurosci, 2009, 12(10):1300-1307.

[4] Robyn J Barst. PDGF signaling in pulmonary arterial hypertension [J]. J Clin Invest, 2005, 115(10): 2691-2694.

[5] Li ZC, Zhang FQ, Song JC, et al. Therapeutic effects of DCDDP, a calcium channel blocker, on chronic pulmonary hypertension in rat [J]. J Appl Physiol, 2002, 92(3): 997-1003.

[6] Koichiro Mukai, Hiroyuki Matsushima, Yasuo Ishii, et al. Effects of calcium on lens epithelial cells in rabbits [J]. Nihon Ganka Gakkai Zasshi, 2006, 110(5):361-369.

[7] Yang Xiao-Ru, Lin Mo-Jun, Lionel S McIntosh, et al. Functional expression of transient receptor potential melastatin- and vanilloid-related channels in pulmonary arterial and aortic smooth muscle [J]. Am J Physiol Lung Cell Mol Physiol, 2006, 290(6): L1267-L1276.

[8] Gao Hai-xia, Chen Xing-juan, Du Xiao-na, et al. EGF enhances the migration of cancer cells by up-regulation of TRPM7 [J]. Cell Calcium, 2011, 50(6): 559-568.

[9] Sidney S Sobin, Peter C Chen. Ultrastructural changes in the pulmonary arterioles in acute hypoxic pulmonary hypertension in the rat [J]. High Alt Med Biol, 2000, 1(4): 311-322.

[10] Emin Gurbanov, Shiliang X. The key role of apoptosis in the pathogenesis and treatment of pulmonary hypertension [J]. Eur J Cardiothorac Surg, 2006, 30(3): 499-507.

[11] Yoshiyuki Tanabe, Maki Saito, Akiko Ueno, et al. Mechanical stretch augments PDGF receptor beta expression and protein tyrosine phosphorylation in pulmonary artery tissue and smooth muscle cells [J]. Mol Cell Biochem, 2000, 215(1-2): 103-113.

[12] Bradford C Berk. Vascular smooth muscle growth: autocrine growth mechanisms [J]. Physiol Rev, 2001, 81(3): 999-1030.

Transient Receptor Potential Melastatin 7 Involved in the Proliferation Induced by Platelet Derived Growth Factor and 5-HT in Pulmonary Artery Smooth Muscle Cells of Rates

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles0

Recommended Articles

Metrics