%A ZHANG Dan-shen %T Establishment about several anti-oxidant models of microplate quantification suitable for drug-screening and their stability testing %0 Journal Article %D 2011 %J ACTA NEUROPHARMACOLOGICA %R %P 45-51 %V 1 %N 1 %U {http://actanp.hebeinu.edu.cn/CN/abstract/article_7.shtml} %8 2011-02-26 %X
Objective:To establish a series of anti-oxidant models of microplate quantification suitable for the drug screening.
Methods:By using SpectraMax M5 continuous spectrum enzyme sign reflectoscope reflector，the anti-oxidant microplate quantification experiment modles of anti-DPPH oxidation activity，elimination hydroxy free radical，reduction ability determination，and lipid peroxidation were established.
Results:All of the anti-oxidant microplate quantification experiment modles had more common characteristices of the good stability，the fewer reagent types and quantity，simple operation and step，the good repeatability，and so on. Relatively speaking，the two anti-oxidant modles of anti-DPPH oxidation activity，reduction ability determination had more advantage in these experiment modles，and had more suitable for initially screening experiment of the anti-oxidant drug as basic research.
Conclusion:These four modles of the anti-oxidant microplate quantification may be widely applied in anti-drug screening，especially high-throughput screening research.