%A ZUO Ya-qi, GAO Zhi-hong, PANG Ce, SUN Zhuan-you, LI Fang, ZHEN Pan
%T Optimized Extraction of Mulberrin from Morus Notabilis Schneid
%0 Journal Article
%D 2020
%J ACTA NEUROPHARMACOLOGICA
%R 10.3969/j.issn.2095-1396.2020.01.005
%P 20-23
%V 10
%N 1
%U {http://actanp.hebeinu.edu.cn/CN/abstract/article_708.shtml}
%8 2020-02-26
%X
Objective:To investigate the optimum extraction technology of mulberrin from Morus notabilis Schneid. Methods:Taking the content of mulberrin as an index,the effects of ethanol concentration,extraction time,solvent multiple,extraction times on the extraction efficiency of mulberrin was investigated by 4-Factor-3-Level orthogonal experiment. HPLC was used to detect the extraction efficiency of mulberrin on YMC-Pack ODS-A column (4.6 mm×250 mm,5 μm) with water- methanol (20:80) as mobile phase. The flow rate was 1.0 mL·min-1,the column temperature was 25 ℃ and the wavelength of UV detector was 268 nm. Results:The optimum extraction technology:before extraction,Morus notabilis was soaked in extraction solvent for 3.0 h,and extracted with reflux for 3 times,each extraction time was 0.5 h,and the extraction solvent multiple was 20 times. Under optimal extraction conditions,the content of mulberry in Morus notabilis Schneid was 1.510 mg·g-1. Conclusion:Ethanol concentration,solvent multiple and extraction times all had significant effects on the extraction efficiency of mulberry,and the extraction time didn’t have significant influences on the extraction efficiency of mulberry.