%A ZHAO Jun,WANG Jin-hui %T
In VivoApplication of Two-photon Microscopy in Neuropharmacological Research%0 Journal Article %D 2012 %J ACTA NEUROPHARMACOLOGICA %R %P 45-64 %V 2 %N 1 %U {http://actanp.hebeinu.edu.cn/CN/abstract/article_72.shtml} %8 2012-02-26 %X Two-photon microscope is an useful and advanced tool for noninvasive deep fluorescence imaging in the intact brain tissue of living animals. Due to nonlinear two-photon effects, two-photon microscope enables long-term imaging
in vivowith deeper detection, higher signal-to-noise ratio and lower photodamage, compared to wide-field and confocal microscopy. Two-photon microscopy can provide high-resolution images to study cellular and subcellular structure and function, including morphology, mobility and intracellular ions of cells. On the other hand, large scale two-photon imaging of cell population reveals the network construction and activity dynamics with single-cell resolution, which makes two-photon microscopy a high throughput tool in system pharmacology. Moreover, two-photon microscopy can offer some precise optical operations, such as photolysis, photoactivation, phototransfection and photodamage. Here, we give an introduction to the principles of two-photon microscopy and its
in vivoapplications in neuroscience and neuropharmacology researches.