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Neuroprotective Effect of S14G-Humanin on SH-SY5Y Cells induced by Oxygen Glucose Deprivation/Reoxygenation SUN Li, ZHANG Xiao-ying, GAO Guang-sheng 2022, 12 (2): 1. DOI:10.3969/j.issn.2095-1396.2022.02.001 Abstract( 8) PDF(4258KB) ( 1) Objective：To establish an in vitro oxygen glucose deprivation/reoxygenation （OGD/R） model using SH-SY5Y neuroblastoma cells to mimic the in vitro ischemia/reperfusion injury in stroke. To evaluate the potential neuroprotective effect of S14G-humanin （HNG） from ischemia/reperfusion in vitro，and further to identify the molecular mechanism underlying the protection，so as to provide a theoretical basis for the treatment of cerebral ischemia/reperfusion injury with HNG. Methods：SH-SY5Y cells were divided into control group，oxygen-glucose deprivation reperfusion OGD/R model group and inhibitor group. The present study established an in vitro OGD/R model using SH-SY5Y neuroblastoma cells by three gas incubator. HNG and FLLL32，an inhibitor of JAK2/STAT3 signal，were added to the cells in indicated experiments. CCK8 kit was used to detect the cell viability，and flow cytometry was used to detect the cell apoptosis rate. The expression of JAK2，p-STAT3（Y705） and p-STAT3（S727） were analyzed by Western Blot. Results：The results showed that OGD/R resulted in a significant decrease in the survival rate of SH-SY5Y cells （P<0.01） and a significant increase in the apoptosis rate of SH-SY5Y cells （ P<0.01）. SH-SY5Y cells incubated 0.1，1，5 or 10 μg·L -1HNG showed higher cell survival （ P<0.01） and significantly decreased apoptosis （ P<0.01） compared with the cells without HNG treatment in OGD/R processes，with the most evident effects at 1 μg·L -1HNG. OGD/R lowered protein levels of JAK2 （ P<0.01） and p-STAT3 at Y705 site （ P<0.01），compared to control. Adding HNG to the cells increased protein levels of JAK2 （ P<0.01） and p-STAT3 （Y705）（ P<0.01） in OGD/R conditions. Moreover，protein levels of JAK2（ P<0.01），p-STAT3 （Y705）（ P<0.01） and p-STAT3（ S727）（ P<0.05） had notable increase at values of 1 and 5 μg·L -1HNG in OGD/R conditions. Co-treatment with HNG and FLLL32 was unable to restore the cell viability and abolishing the inhibition of apoptosis by HNG（ P>0.05）. HNG inhibited OGD/ R-induced reduction of JAK2，p-STAT3（Y705） and p-STAT3（S727） protein expression， whereas the inhibitory effect was not observed with co-treatment with HNG and FLLL32. Conclusion：These data collectively indicate that HNG has neuroprotective effects against OGD/ R by activating JAK2/STAT3 signaling and suggest that HNG may be a promising drug for the treatment of stroke. Related Articles|Metrics

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Protective Effect of Huangjiaruangan Granule on Acute Liver Injury and Liver Fibrosis in Mice GAO Xiao-xin, LI Xue-mei, WANG Jia-shuai, et al 2022, 12 (2): 9. DOI:10.3969/j.issn.2095-1396.2022.02.002 Abstract( 5) PDF(1142KB) ( 0) Objective：To study the protective effect of Huangjiaruangan granule on the acute liver injury induced by D-galactosamine （D-Gal N） and the liver fibrosis induced by Con A. Methods：Test method for protective effect of acute liver injury：Eighty-four ICR mice were randomly divided into negative control group，model group，positive drug group，and high-dose， medium-dose and low-dose huangjiarangan granule groups. The negative control group and model group were given the same volume of deionized water by intragastric administration，once a day for 14 consecutive days. On day 13 after administration，all animals except the negative control group were intraperitoneally injected with D-Gal N to establish acute liver injury model. Blood samples were collected and necrotized 60 minutes after the last administration. Test method for protective effect of liver fibrosis：96 BALB/ c mice were randomly divided into negative control group，model group，positive drug group，Huangjiarangan granule high-dose，medium-dose and low-dose groups. Animals except the negative control group were injected with Con A according to tail vein，and animals in the negative control group were injected with sterile PBS of the same volume，once a week，7 times in total. On the day of the fourth injection，each group was given intragastric administration，once a day，for 4 weeks. The negative control group and the model group were given intragastric administration of the same volume of deionized water. Blood samples were collected and examined 24 hours after the last injection. Serum ALT and AST were measured in both tests，liver and spleen were collected and weighed to calculate organ index，and pathological changes of liver tissues were observed. Results：In the protective effect test of acute liver injury，the serum ALT and AST of each administration group were significantly lower than that of the model group，and the spleen index of each administration group was significantly reduced，and the degree of liver injury was significantly reduced. In the liver fibrosis protective effect test，the serum ALT and AST in Huangjiaruangan granules high-dose and medium-dose groups were significantly lower than that in the model group，and the liver index in the mediumdose group was significantly lower. Conclusion：Huangjiaruangan granule has a certain protective effect on acute liver injury and liver fibrosis in mice. Related Articles|Metrics

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Internal Quality Control and External Quality Assessment of SAT Test for Mycoplasma Pneumoniae WANG Bin, XU Jia-yin, LI Ya-di, et al 2022, 12 (2): 14. DOI:10.3969/j.issn.2095-1396.2022.02.003 Abstract( 17) PDF(1480KB) ( 0) Objective：The method of testing Mycoplasma pneumoniae by SAT was used to carry out Internal Quality Control and External Quality Assessment in Zhangjiakou Jianyuan Medical Laboratory，so as to find out the deficiencies of the laboratory and make adjustments to improve the laboratory quality. Methods：Mycoplasma pneumoniae （MP） quality control products were diluted 10 times as positive control，and then diluted 10 times，100 times and 1 000 times on the basis of the positive control，in which the dilution of 100 times and 1 000 times was repeated for four times. Simultaneous Amplification and Testing （SAT） of RNA was used to observe the curve，sensitivity and precision of detection under the same conditions as negative control and positive control. In addition，multiple MP samples provided by the Molecular Laboratory of the Pediatric Research Institute of Tianjin Children’s Hospital were carried out blind RNA testing. Finally，the test results of the two laboratories were compared to make an accurate evaluation of the laboratory. Results：The quality control products with 10 times，100 times and 1 000 times dilution were all positive，and the CT values were all less than 30 cycles. Observe the curves of four repeat tubes of 100 times diluted quality control products and the curves of four repeat tubes of 1 000 times diluted quality control products，all of which are “S” shaped curves. The results of MP sample RNA blind test were positive for the first one and negative for the rest，which were consistent with the results provided by the Molecular Laboratory of Pediatric Research Institute of Tianjin Children’s Hospital. Conclusion：IQC test shows that the laboratory has good sensitivity and precision，and EQA test shows that the laboratory has very high accuracy，which all meet the requirements of relevant standards for the laboratory，making the test results more meaningful. Related Articles|Metrics

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Effects of Peony Licorice Decoction Extract on Oxidative Stress and Inflammatory Response of Cardiomyocytes induced by Hydrogen Peroxide LIU Jin-jie, HE Wei, PENG Xin-ru, et al 2022, 12 (2): 21. DOI:10.3969/j.issn.2095-1396.2022.02.004 Abstract( 16) PDF(1368KB) ( 2) Objective：To investigate the effect of Peony licorice decoction extract （SYG） on oxidative stress and inflammatory response induced by hydrogen peroxide （H 2O 2） on myocardial cells. Methods：Myocardial cells were divided into five groups，respectively normal group，model group （200 μmol·L -1H 2 O 2） and SYG groups （150，100，50 μg·mL -1），and after the intervention of Peony licorice decoction for 24 h，H 2 O 2was treated for 2 h，then MTT method was used to detect cell proliferation ability，determination of superoxide dismutase （SOD），catalase （CAT），lactate dehydrogenase （LDH） and malondialdehyde （MDA） by UV spectrophotometry. RT-PCR was used to evaluate the inflammatory factor tumor necrosis factor-α（ TNF-α），inducible nitric oxide synthase （iNOS），cyclooxygenase-2 （COX-2），and nuclear factor kappa-B （NF-κB）in myocardial cells. Results：H 2 O 2induction could significantly reduce the contents of SOD and CAT，and significantly increase the contents of MDA and LDH，while SYG could significantly improve the above phenomena，and SYG could inhibit the expression of TNF-α，iNOS，COX- 2 and NF-κB. Conclusion：SYG can inhibit the oxidative stress and inflammatory response of myocardial cells induced by H 2 O 2，and has a certain protective effect on myocardial ischemia. It is speculated that its anti-inflammatory mechanism may be related to the inhibition of TNF-α to activate NF-κB pathway to inhibit the expression of iNOS and COX-2，but the specific mechanism still needs to be further clarified. Related Articles|Metrics

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Metagenomic Next‐Generation Sequencing and Traditional Way Contrast in Centralnervous System Infectious Diseases ZHANG Xiao-jie, FAN Lei, PAN Yan-ting 2022, 12 (2): 27. DOI:10.3969/j.issn.2095-1396.2022.02.005 Abstract( 7) PDF(1155KB) ( 0) Objective：To assess the clinical value of metagenomic next-generation sequencing （mNGS） of cerebrospinal fluid in diagnosis of neurological infectious disease. Methods：Patients who were clinically diagnosed with neurological infectious treated in Department of Neurology，Affiliated Hospital of Hebei North University from May 2019 to May 2021 were retrospectively analyzed intotal 60，including the data of mNGS and traditional laboratory test of pathogens. Results：It was including 42 bacteria infection，16 viral infection and 2 parasitic infection. The accurate rate of bacteria，viral and parasitic by mNGS was 95.24%， 68.75% and 50%. The AUC of mNGS was 0.705 and the diagnostic value was medium. Compared with the diagnostic reference line with AUC=0.5，the difference was statistically significant （ P<0.05）. Conclusion：ThemNGS is more sensitive evaluating the neurological infectious. It has advantages in accurate diagnosis of neurological infectious disease. Related Articles|Metrics

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Clinical Study on the Treatment of Pruritus in Peritoneal Dialysis Patients with Runfu Zhiyang Decoction LI Zhen-wen, ZHANG Ya-nan, SUN Hong-sheng 2022, 12 (2): 31. DOI:10.3969/j.issn.2095-1396.2022.02.006 Abstract( 13) PDF(1125KB) ( 3) Objective：To investigate the clinical effect of Runfu Zhiyang Decoction in the treatment of peritoneal dialysis patients complicated with skin pruritus. Methods：A retrospective analysis was used to select 40 patients with pruritus who regularly underwent peritoneal dialysis in Shijiazhuang Hospital of Traditional Chinese Medicine from September 2020 to January 2022 as the research objects. All patients were treated with Moisturizing and relieving pruritus prescription fumigation and washing. After 4 weeks of treatment，the visual analogue scale （VAS） and TCM syndrome score scale were used to evaluate the skin pruritus before and after treatment. Results：The VAS score and TCM syndrome score after treatment were lower than those before treatment，and the difference was statistically significant （ P<0.05）. The total effective rate was 82.5%. Conclusion：Runfu Zhiyang Decoction in the treatment of peritoneal dialysis patients complicated with skin pruritus has obvious effect，can reduce the degree of pruritus， improve the psychological state and quality of life of patients，high safety，worthy of clinical promotion. Related Articles|Metrics

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Denosumab in the Treatment of Rheumatoid Arthritis ZHANG Mei, WEI Wei 2022, 12 (2): 35. DOI:10.3969/j.issn.2095-1396.2022.02.007 Abstract( 66) PDF(1144KB) ( 0) Osteoporosis is a common complication of rheumatoid arthritis （RA）. Denosumab，a human antibody against receptor activator of nuclear factor-kappa B ligand，can suppress the differentiation and activation of osteoclast，increase bone mineral density （BMD）， and reduce the incidence of fracture. Denosumab can be used in the treatment of rheumatoid arthritis. In the patients with RA，it can efficiently increase bone mineral density，suppress the progression of bone erosion，but it does not affect synovitis and disease activity. The combination of denosumab and disease-modifying anti-rheumatic drugs，can inhibit bone erosion synergistically with good tolerance and no increases in infection rates. We should concern about rare side effects，such as osteonecrosis of the jaw and atypical femoral fracture. Calcium and vitamin D supplementation with denosumab treatment is beneficial to prevent hypocalcemia. We should pay attention to the risk of bone mineral density decline and vertebral fractures after drug discontinuation. Denosumab discontinuation might be considered under the guidance of doctors. Administration of a bisphosphonate after denosumab discontinuation is recommended. Related Articles|Metrics

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Research Progress of Agrimony in the Treatment of Intestinal Diseases ZHAO Hui-qiao, SHI Tong-tong, LI Zhong-ping, et al 2022, 12 (2): 42. DOI:10.3969/j.issn.2095-1396.2022.02.008 Abstract( 8) PDF(1084KB) ( 0) By collecting recent literature on the chemical constituents of Agrimony， potential active ingredients for intestinal diseases，and the clinical application of Agrimony，we found that Agrimony can not only treat hemoptysis，hematemesis，metrorrhagia and other types of hemorrhage，but also treat inflammatory bowel disease，irritable bowel syndrome，bacillary dysentery and other intestinal diseases. In this paper，a review is carried out on the basis of this research，in order to provide more theoretical basis for the treatment of intestinal diseases by Agrimony. Related Articles|Metrics

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Research Progress in Migraine Combined with Patent Oval Foramen ZHAO Bo, YU Ai-mei, ZOU Yu-an 2022, 12 (2): 47. DOI:10.3969/j.issn.2095-1396.2022.02.009 Abstract( 5) PDF(1144KB) ( 0) Migraine is a disabling chronic neurovascular disease characterized by severe hemiplegic headache attacks. Migraine attacks are intensely painful，and when they occur， most activities of daily living and work can no longer be carried out normally，productivity is significantly reduced，and healthcare-related costs are significantly increased. Migraine ranks third among the most common diseases in the world（after dental caries and tension headaches）， and ranks eighth in disability due to disease，with high morbidity，high disease burden，and wide impact. Significant adverse effects on the patient's quality of life. Related Articles|Metrics

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Study on the Influencing Factors and Related Mechanisms of Dyskinesia in Parkinson’s Disease DONG Lu-lu, WANG Lu-lu, CHEN Jing-hong, et al 2022, 12 (2): 53. DOI:10.3969/j.issn.2095-1396.2022.02.010 Abstract( 11) PDF(1091KB) ( 0) Long-term administration of levodopa to restore dopamine levels in the brain has long been the gold standard treatment for Parkinson’s disease （PD）. Levodopa supplementation improves the motor symptoms of PD，but the effect of long-term medication on levodopa-induced dyskinesias （LID） is still a controversial and urgent problem to be solved clinically. At present，there are different analyses on the risk factors of LID caused by levodopa， and many factors affect each other. This article will review the incidence and influencing factors of PD dyskinesia，so as to avoid intervention of risk factors in the process of diagnosis and treatment，prevent and reduce the occurrence of LID as early as possible. Related Articles|Metrics

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Discussion on the Treatment of Type 2 Diabetes from Liver and Spleen SONG Qiu-jing, WANG Juan 2022, 12 (2): 58. DOI:10.3969/j.issn.2095-1396.2022.02.011 Abstract( 13) PDF(1124KB) ( 0) Type 2 diabetes is a genetically predisposed endocrine metabolic disease caused by multiple factors. The disease is widespread worldwide and seriously endangers human health. At present，the pathogenesis of modern medicine for this disease has not been fully understood， clinical treatment of this disease is mostly to enhance the sensitivity of islet β cells or promote insulin secretion to stabilize glucose，including diet exercise therapy，oral hypoglycemic drug therapy，insulin therapy and so on. Traditional medicine believes that type 2 diabetes belongs to the category of Xiaoke，the disease is located in the lungs，stomach and kidneys，and its basic disease mechanism is yin deficiency internal dry heat，and the system of triple Xiao is adopted when dialectics. However，with the rapid development of the times and the changes in the lifestyle of modern people，the existing treatment methods of traditional Chinese and Western medicine have been unable to meet the needs of clinical type 2 diabetes patients in an all-round and multi-level manner，so it is imperative to seek safe and efficient treatment. Whether from the perspective of basic theory，molecular mechanism，animal experiment or clinical research，there are unique advantages in the treatment of type 2 diabetes from the liver and spleen，which have gradually become a hot topic in today’s research. This article reviews the research progress of traditional Chinese and Western medicine in the treatment of type 2 diabetes from the liver and spleen，so as to provide new treatment ideas for the clinical treatment of type 2 diabetes. Related Articles|Metrics