神经药理学报

神经药理学报››2021,Vol. 11››Issue (2): 5-10.DOI:10.3969/j.issn.2095-1396.2021.02.002

• 研究论文 •上一篇下一篇

穿龙薯蓣皂苷元对CIA小鼠CD4+CD25-T分化为Treg细胞的诱导作用

梁秀军，于永洲，夏东帅，等

  1. 1承德医学院，承德，067000，中国

    2河北省神经损伤与修复重点实验室，承德，067000，中国

    3承德市中心医院，承德，067000，中国

  • 出版日期:2021-04-26发布日期:2021-04-26
  • 作者简介:梁秀军，女，讲师；研究方向：中药免疫；E-mail：149304426@qq.com
  • 基金资助:

    河北省高等学校科学技术研究项目（No.QN2016031），河北省中医药管理局科研计划项目（No.2015155），河北省科技厅中央引导地方科技发展基金项目（No.216Z2501G）

Differentiation of CD4+CD25-T Cells to Regulatory T Cells induced by Diosgenin in CIA Mice

LIANG Xiu-jun，YU Yong-zhou，XIA Dong-shuai，GAO Ya-xian，SUN Tong-you

  1. 1 Chengde Medical University，Chengde，067000，China

    2 Hebei Key Laboratory of Nerve Injury and Repair，Chengde 067000，China

    3 Chengde Central Hospital，Chengde，067000，China

  • Online:2021-04-26Published:2021-04-26
  • About author:梁秀军，女，讲师；研究方向：中药免疫；E-mail：149304426@qq.com
  • Supported by:
    河北省高等学校科学技术研究项目（ No.QN2016031 ），河北省中医药管理局科研计划项目（ No.2015155 ），河北省科技厅中央 引导地方科技发展基金项目（ No.216Z2501G ）

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摘要：

目的：探讨穿龙薯蓣皂苷元诱导胶原性关节炎（collagen induced arthritis，CIA）小鼠CD4+CD25-T细胞向调节性T细胞（regulatory T cells，Treg）转化的影响。方法：采用免疫磁性细胞分离法（magnetic activated cell sorting，MACS）分离CIA小鼠的CD4+CD25-T细胞。通过不同浓度（25、12.5、6.25μmol·L-1）的穿龙薯蓣皂苷元诱导CD4+CD25-T细胞向Treg细胞分化，用FCM检测Treg细胞比率，Real-Time PCR法检测Foxp3 mRNA的水平，ELISA法检测培养上清白细胞介素10（interleukin 10，IL-10）的含量。结果：免疫磁性细胞分离法分选出的CD4+CD25-T细胞纯度达93.89%。与对照组相比，穿龙薯蓣皂苷元给药组Treg细胞比率明显上调（P<0.05），Foxp3 mRNA的表达水平和培养上清中IL-10的水平均明显增加（P<0.05）。结论：穿龙薯蓣皂苷元可以诱导

CIA小鼠CD4+CD25-T细胞向Treg细胞分化，并促进Foxp3的表达。

关键词:

font-family:Helvetica,">font-family:Times,">CIA模型；薯蓣皂苷元；调节性font-family:Times,">T细胞

Abstract:

Objective：To investigate the roles of diosgenin in the differentiation of regulatory T cells from CD4+CD25-T cells in collagen induced arthritis mice. Methods：

CD4+CD25-T cells of CIA mice isolated by immunomagnetic beads on magnetic activated cell sorting device were co-cultured with different concentrations of diosgenin（25、12.5、6.25μmol·L-1）for 48 hours. The ratio of Treg cells was tested by flow cytometry. The level of Foxp3 mRNA was measured by Real-Time PCR. The level of serum IL-10 was detected by ELISA. Results：This study separated CD4+CD25-T cells of 93.89% purity. The ratio of Treg cells，the level of IL-10 and Foxp3 mRNA were increased significantly in diosgenin groups compared with those in control group（P<0.05）. Conclusion：Diosgenin may induce the differentiation of CD4+CD25-T cells to regulatory T cells in CIA mice and up-regulate the expression of Foxp3.

Key words:

font-family:Times,"> CIA modelfont-family:Helvetica,">；diosgeninfont-family:Helvetica,">；regulatory T cells

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